肠道微生物群移植和益生菌补充剂对妊娠期糖尿病大鼠抗炎症、抗氧化及妊娠结局的影响  

Effects of fecal microbiota transplantation and probiotic supplementation on antiinflammation,anti-oxidation and pregnancy outcome in gestational diabetic rats

作　　者：郑文璐 李莉[2] 王昆 马依拉·买买提[2] 赵效国 ZHENG Wenlu;LI Li;WANG Kun;Mayla MEMET;ZHAO Xiaoguo(Department of Toxicology,College of Public Health,Xinjiang Medical University,Urumqi,Xinjiang 830054,China;不详)

机构地区：[1]新疆医科大学公共卫生学院卫生毒理学教研室,新疆乌鲁木齐830054 [2]新疆医科大学第一附属医院临床营养科 [3]新疆医科大学实验动物中心

出　　处：《中国微生态学杂志》2024年第6期657-664,670,共9页Chinese Journal of Microecology

基　　金：新疆维吾尔自治区自然科学基金(2022D01C444)。

摘　　要：目的 探讨肠道微生物群移植(FMT)和益生菌补充剂对妊娠期糖尿病(GDM)大鼠抗炎症、抗氧化及妊娠结局的影响,为临床应用提供基础依据。方法 取50只SD雌性大鼠和25只SD雄性大鼠,2∶1合笼,交配成功后随机抽取10只雌鼠为对照组,其余雌鼠腹腔注射链脲佐菌素建立GDM大鼠模型,随机分为模型组、益生菌组、FMT组、FMT+益生菌组,每组10只。FMT组给予10 mL/kg粪便混悬液灌胃,益生菌组给予359.708 mg/kg益生菌制剂灌胃,FMT+益生菌组给予10 mL/kg粪便混悬液和359.708 mg/kg益生菌制剂灌胃,模型组、对照组进行等量PBS缓冲液灌胃,每天一次,14 d后检测大鼠血清中空腹血糖(FBG)、空腹胰岛素(FINS)、总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)、肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素6(IL-6)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)水平,记录大鼠的胎鼠体重、胎盘重量及胎鼠存活数。结果 模型组大鼠的胎鼠体重、胎盘重量,血清中FBG、TC、TG、LDL、FINS、TNF-α、IL-1β、IL-6水平,肠道菌群弯曲菌门、脱硫杆菌门相对丰度均显著高于对照组(均P<0.05);模型组大鼠活胎鼠数量,血清中HDL水平,SOD、GSH-Px活性,肠道菌群Ace指数、Chao1指数,Lachnospiraceae NK4A136 group、未分类毛螺菌属相对丰度均显著低于对照组(均P<0.05);FMT组、益生菌组、FMT+益生菌组大鼠的胎鼠体重、胎盘重量,血清中FBG、 TC、 TG、 LDL、 FINS、 TNF-α、 IL-1β、 IL-6水平均显著低于模型组(均P<0.05);FMT组、FMT+益生菌组大鼠肠道菌群Ace指数、Chao1指数及弯曲菌门相对丰度均显著高于模型组(均P<0.05);益生菌组Ace指数、Chao1指数均显著低于对照组(均P<0.05),拟杆菌门、放线菌门、Lachnospiraceae NK4A136 group相对丰度均显著高于模型组(均P<0.05),厚壁菌门相对丰度显著低于模型组(P<0.05);FMT组、�Objective To observe the effects of fecal microbiota transplantation(FMT)and probiotic supplementation on anti-inflammation,antioxidant activity and pregnancy outcomes in gestational diabetes mellitus(GDM)rats.Methods Fifty female SD rats and twenty-five male ones were paired in a 2∶1 ratio.After successful mating,ten female rats were randomly selected as the control group.The remaining female rats were intraperitoneally injected with STZ to establish the GDM rat models,and were randomly divided into the model group,probiotic group,FMT group,and combination of FMT and probiotic group.The FMT group received 10 mL/kg of fecal suspension by gavage,the probiotic group was given 359.708 mg/kg of probiotic formulation,while the combination group received both 10 mL/kg of fecal suspension and 359.708 mg/kg of probiotic formulation.The model and control groups were given an equivalent volume of PBS buffer.Each treatment was administered once daily for 14 days.Afterward,serum levels of fasting blood glucose(FBG),fasting insulin(FINS),total cholesterol(TC),triglycerides(TG),low-density lipoprotein(LDL),high-density lipoprotein(HDL),tumor necrosis factor-alpha(TNF-α),interleukin-1β(IL-1β),interleukin-6(IL-6),superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),and malondialdehyde(MDA)were measured.The weight of rat placenta,number of survival fetuses and their weights were also recorded.Results In the model group,the weights of fetuses and placentas,and the levels of FBG,TC,TG,LDL,FINS,TNF-α,IL-1βand IL-6 in serum,as well as the relative abundances of Bacteroidetes and Desulfovibrionaceae in intestinal flora were significantly higher,while the number of survival fetuses,serum HDL level,activities of SOD and GSH-Px,Ace index,Chao1 index,and abundances of Lachnospiraceae NK4A136 group and unclassified Clostridiaceae abundance in intestinal flora were significantly lower than those in the control group,respectively(all P<0.05).In the FMT group,probiotic group and combination group,the weights of fetuses and placenta

关 键 词：妊娠期糖尿病 肠道微生物群移植 益生菌补充剂 妊娠结局 

分 类 号：Q939.93[生物学—微生物学] R173[医药卫生—妇幼卫生保健]