Structure-guided isolation of anti-neuroinflammatory sesquiterpene coumarins from Ferula sinkiangensis  

作　　者：DANG Wen GUO Tingting ZHOU Di MENG Qingqi FANG Mingxia CHEN Gang LIN Bin HOU Yue LI Ning 

机构地区：[1]School of Traditional Chinese Materia Medica,Key Laboratory of Innovative Traditional Chinese Medicine for Major Chronic Diseases of Liaoning Province,Key Laboratory for TCM Material Basis Study and Innovative Drug Development of Shenyang City,Shenyang Pharmaceutical University,Shenyang 110016,China [2]College of Life and Health Sciences,Northeastern University,Shenyang 110004,China [3]School of Pharmaceutical Engineering,Shenyang Pharmaceutical University,Shenyang 110016,China

出　　处：《Chinese Journal of Natural Medicines》2024年第7期643-653,共11页中国天然药物（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.U1903122);the Scientific Research Fund of Liaoning Province Education Department(No.LJKZ0945);the Natural Science Foundation of Liaoning Province(No.2022-MS-242).

摘　　要：The resin of Ferula sinkiangensis has been traditionally utilized for treating gastrointestinal disorders,inflammation,tumors,various cancers,and alopecia areata.The primary bioactive constituents,sesquiterpene coumarins,have demonstrated notable therapeutic potential against neuroinflammation.In this study,a structure-guided fractionation method was used to isolate nine novel sesquiterpene coumarins from the resin of F.sinkiangensis.These compounds were characterized and structurally elucidated using comprehensive physicochemical and spectroscopic techniques,including calculated electronic circular dichroism(ECD).Anti-neuroinflammatory assays revealed that compounds 2,3,and 6 significantly inhibited nitric oxide(NO)production in lipopolysaccharide(LPS)-stimulated BV2 microglial cells,with IC_(50)values ranging from 1.63 to 12.25μmol·L^(−1).

关 键 词：Ferula sinkiangensis Sesquiterpene coumarins Structure elucidation Anti-neuroinflammatory activity 

分 类 号：R284.1[医药卫生—中药学] R965[医药卫生—中医学]