毛细管电泳-激光诱导荧光测序仪偏振特性研究  

Research on polarization characteristics of capillary electrophoresis with laser-induced fluorescence sequencer

作　　者：曾涵智 孙丹[2] 苑帅康 张旭升[1] ZENG Hanzhi;SUN Dan;YUAN Shuaikang;ZHANG Xusheng(School of Optics and Photonics,Beijing Institute of Technology,Beijing 100081,China;First Research Institute of the Ministry of Public Security of PRC,Beijing 100044,China)

机构地区：[1]北京理工大学光电学院,北京100081 [2]公安部第一研究所,北京100044

出　　处：《光学技术》2024年第4期477-483,共7页Optical Technique

基　　金：国家重点研发计划项目(2021YFC3300100)。

摘　　要：基于毛细管电泳-激光诱导荧光原理测序仪中,激发光偏振态对拉曼光和荧光的激发效率有影响。针对该特性进行了深入研究,通过理论建模仿真和偏振光线追迹分析了光学系统对于入射光偏振态的影响,并且搭建了实验系统研究了激发光偏振方向对于激发结果的影响。实验结果表明,作为筛分介质的POP-7聚合物的拉曼散射光强度会随激发光偏振方向的改变而产生明显的变化,波动值高达50%以上,且此波动值随着激发光强度降低而相应减小;对于多色荧光染料,在激发过程中表现出完全的随机性,不同浓度梯度下各色荧光对于激发光偏振方向改变具有各自不同的响应度,但均未表现出与激发光偏振方向显著的相关性。In the sequencer based on the principle of capillary electrophoresis with laser-induced fluorescence,the polarization direction of the excitation light has an impact on the excitation efficiency of Raman scattering and fluorescence.In-depth research on this characteristic is conducted,the influence of optical systems on the polarization state of incident light through theoretical simulation modeling and polarization ray tracing is analyzed,and an experimental system to study the influence of polarization direction of excitation on the excitation results is built,in this paper.The experimental results show that the Raman scattering light intensity of POP-7polymer as a screening medium will undergo significant changes with the change of polarization direction of excitation light,with a fluctuation value of over 50%,and this fluctuation value will correspondingly decrease with the decrease of excitation light intensity;For multi-color fluorescent dyes,they exhibit complete randomness during the excitation process.Under different concentration gradients,each color of fluorescence has its own response to changes in the polarization direction of the excitation light,but none of them show significant correlation with the polarization direction of the excitation light.

关 键 词：偏振光学 激光诱导荧光 拉曼光谱 激发效率 

分 类 号：TN29[电子电信—物理电子学]