基于光纤SPR的表面增强荧光特性研究  

Research on surface-enhanced fluorescence characteristics of optical fiber SPR

作　　者：赵静涛 张振兴 孙响 孔梅[1] 张荣[1] ZHAO Jingtao;ZHANG Zhenxing;SUN Xiang;KONG Mei;ZHANG Rong(School of Physics,Changchun University of Science and Technology,Changchun 130013,China)

机构地区：[1]长春理工大学物理学院光波导实验室,吉林长春130013

出　　处：《光学技术》2024年第4期508-512,共5页Optical Technique

摘　　要：随着表面等离激元理论和集成光学技术的研究和发展,在生物医学检测领域,通过对光学传感器敏感膜表面增强荧光特性的研究,从而提高检测灵敏度的方式越来越受到关注。提出了一种基于表面增强荧光(SEF)的光纤型荧光传感器,并通过有限元方法进行了分析。仿真结果表明,在金膜厚度为50nm、表面等离子体共振(SPR)波长为566nm时,场增强因子达到最大值12.11,此时荧光增强效果较好。采用磁控溅射法对所设计的光纤传感器进行加工,搭建荧光检测平台并对Alexa Fluor 594羧酸(AF594-COOH)的荧光发射强度进行测试。实验结果表明,镀金多模光纤激发的荧光强度相比于未镀金光纤增强了3.35倍。With the research and development of surface plasmon theory and integrated optics technology,the way to enhance the detection sensitivity through the study of enhanced fluorescence properties on the surface of the sensitive membrane of optical sensors is getting more and more attention in the field of biomedical detection.An optical fiber-type fluorescence sensor based on surface-enhanced fluorescence(SEF)is proposed and analyzed through the finite element method.The simulation results show that the field enhancement factor reaches a maximum value of 12.11when the gold film thickness is 50nm and the Surface Plasmon Resonance(SPR)wavelength is 566nm,at which time the fluorescence enhancement effect is better.The designed fiber optic sensor was processed by magnetron sputtering method,and the fluorescence detection platform was built and tested for the fluorescence emission intensity of Alexa Fluor 594carboxylic acid(AF594-COOH).The experimental results show that the fluorescence intensity excited by the gold-plated multimode fiber is enhanced by a factor of 3.35compared to that of the non-gold-plated fiber.

关 键 词：表面增强荧光 表面等离子体共振 场增强 Alexa Fluor 594羧酸 

分 类 号：Q433.4[生物学—生理学]