红色板栗和绿色板栗叶片光合特性及色素含量比较  

Comparison of photosynthetic characteristics and pigment content of red Chinese chestnut and green Chinese chestnut leaves

作　　者：杨涌 勾荣鑫 付丽枝 王萌[2,3,4] 王东升 张海娥[2,3,4] YANG Yong;GOU Rongxin;FU Lizhi;WANG Meng;WANG Dongsheng;ZHANG Hai'e(College of Horticulture Science and Technology,Hebei Normal University of Science and Technology,Qinhuangdao 066004;Engineering Research Center of Chestnut Industry Technology, Ministry of Education;Hebei Key Laboratory of Horticultural Germplasm Excavation and Innovative Utilization;Hebei Collaborative Innovation Center of Chestnut Industry)

机构地区：[1]河北科技师范学院园艺科技学院,秦皇岛066004 [2]板栗产业技术教育部工程研究中心 [3]河北省特色园艺种质挖掘与创新利用重点实验室 [4]河北省板栗产业协同创新中心

出　　处：《中国果树》2024年第6期62-67,共6页China Fruits

基　　金：国家重点研发计划项目“主要经济林优质高产新品种创制与精准栽培技术”(2022YFD2200400);河北科技师范学院博士启动基金项目“板栗光效分型及高光效生理机制研究”(2023YB026)。

摘　　要：以红色板栗红栗2号和绿色板栗燕宝为材料,通过Li-6400便携式光合仪测定叶片光合参数日变化以及红色板栗与绿色板栗的光响应曲线,同时使用紫外可见分光光度计测定叶片色素含量,探究红色板栗与绿色板栗叶片光合特性差异。结果表明:两种颜色板栗净光合速率日变化有明显区别,绿色板栗净光合速率为双峰形曲线,13:00时出现“光合午休”现象,而红色板栗为单峰形,无明显“光合午休”现象;红色板栗气孔导度、蒸腾速率、水分利用率日均值均大于绿色板栗,而胞间二氧化碳浓度日均值小于绿色板栗。光响应曲线结果表明,红色板栗有更高的最大净光合速率、光补偿点、暗呼吸速率、光饱和点与表观量子效率,与绿色板栗差异显著。对叶片色素含量进行分析,红色板栗与绿色板栗类胡萝卜素含量无明显差异,但是叶绿素含量和花色素苷含量差异显著。相关性分析结果表明,光响应特征参数中最大净光合速率、光饱和点与花色素苷含量有极显著正相关性,而光补偿点、暗呼吸速率与叶绿素含量呈现极显著或显著负相关。Red Chinese chestnut ‘Hongli 2' and green Chinese chestnut ‘Yanbao' were used as the materials.The photosynthetic parameters for the diurnal variation of the leaves and light response curve with the red Chinese chestnut and green Chinese chestnut were determined through the Li-6400 portable photosynthetic analyzer.Pigment content in the leaves were also determined using ultraviolet spectrophotometer.To explore the differences in the photosynthetic characteristics of the red Chinese chestnut and green Chinese chestnut leaves.The results showed that there is a clear difference in the net photosynthetic rate between the two colors of Chinese chestnuts.The net photosynthetic rate of green Chinese chestnut is a bimodal curve,and the “midday depression of photosynthesis”phenomenon appears at 13:00 o'clock.The red Chinese chestnut is unimodal,with no obvious “midday depression of photosynthesis” phenomenon.The daily mean of stomatal conductance,transpiration rate and water use efficiency of red Chinese chestnut was greater than that of green Chinese chestnut,while the daily mean of intercellular carbon dioxide concentration was less than that of green Chinese chestnut.The light response curve indicates that red Chinese chestnut has a greater maximum net photosynthetic rate,light compensation point,dark respiration rate,light saturation point and apparent quantum efficiency,which is significantly different from green Chinese chestnut.Analysis of leaf pigment content showed no significant difference in carotenoid content between the two colors of Chinese chestnuts.However,the content of chlorophyll and the content of anthocyanin difference varied significantly.The correlation analysis showed that the maximum net photosynthetic rate,the light saturation point of the light response characteristic parameters have a very significant positive correlation with the anthocyanin content.However,the light compensation point and the dark respiration rate showed a very significant or significant negative correlation with

关 键 词：板栗 光合日变化 光响应曲线 花色素苷 叶绿素 

分 类 号：S664.2[农业科学—果树学]