荔枝转录因子LcMYB8的克隆与表达分析  

Cloning and expression analysis of litchi transcription factor LcMYB8

作　　者：石雨雨 卿昊炜 桂秋林 黎明 易晨歆 黄川 陈艳艳 刘红枫 郭慧勤 彭宏祥[1] 丁峰 张树伟[1] SHI Yuyu;QING Haowei;GUI Qiulin;LI Ming;YI Chenxin;HUANG Chuan;CHEN Yanyan;LIU Hongfeng;GUO Huiqin;PENG Hongxiang;DING Feng;ZHANG Shuwei(Guangxi Academy of Agricultural Sciences,Guangxi Crop Genetic Improvement and Biotechnology Laboratory,Nanning 530007;College of Agriculture, Guangxi University;Guangxi Lingshan County Agricultural Science Research Institute)

机构地区：[1]广西农业科学院,广西作物遗传改良生物技术重点开放实验室,南宁530007 [2]广西大学农学院 [3]广西灵山县农业科学研究所

出　　处：《中国果树》2024年第6期68-76,共9页China Fruits

基　　金：国家自然科学基金项目(32060659);广西自然科学基金项目(2024GXNSFFA010016、2022GXNSFDA080010、2022GXNSFAA035605);广西农业科学院科技发展基金(2021YT124);广西科技重大专项(桂科AA23023007)。

摘　　要：为探究LcMYB8转录因子在荔枝成花调控中的作用,以妃子笑荔枝为试验材料,克隆LcMYB8转录因子的序列全长,并对其进行生物信息学分析。通过实时荧光定量PCR技术,分析LcMYB8转录因子在不同器官、年周期以及低温处理中的时空表达模式。结果显示,LcMYB8转录因子全长1749 bp,其中开放阅读框915 bp,共编码304个氨基酸。时空表达模式表明,LcMYB8转录因子可能在荔枝成花和果实发育期起着关键作用。研究结果旨在为荔枝优质新品种培育提供理论指导。To investigate the role of Lc MYB8 transcription factor in the regulation of litchi flowering,this study used ‘Feizixiao' litchi as experimental material,cloned the full length of Lc MYB8 transcription factor sequence,and performed bioinformatics analysis on it.In addition,real-time fluorescence quantitative PCR technology was used to analyze the spatiotemporal expression patterns of Lc MYB8 transcription factor in different tissues and organs,annual cycles and low-temperature treatment.The results showed that the Lc MYB8 transcription factor had a total length of1 749 bp,with an open reading frame of 915 bp,encoding a total of 304 amino acids.The spatiotemporal expression patterns suggest that the Lc MYB8 transcription factor may play a crucial role in the flowering and fruit development stages of litchi.This study aims to provide theoretical guidance for the cultivation of high-quality new varieties of litchi.

关 键 词：荔枝 LcMYB8转录因子 表达模式 成花调控 

分 类 号：S667.1[农业科学—果树学]