TUB2基因作为葡萄白粉病菌DNA条形码的生物信息学分析  

Bioinformatics analysis of TUB2 gene for DNA barcoding of the causal pathogen of powdery mildew on grape

作　　者：何瑞玒 付海滨 尹祺 李鑫 宋晓昕 金璐 李辉 HE Ruihong;FU Haibin;YIN Qi;LI Xin;SONG Xiaoxin;JIN Lu;LI Hui(Ningxia Technical College of Wine and Desertification Prevention,Yinchuan 750001;Integrated Technical Service Center of Donggang Customs House;Shenyang Customs Technical Center;Dalian Customs Technical Center)

机构地区：[1]宁夏葡萄酒与防沙治沙职业技术学院,银川750001 [2]东港海关综合技术服务中心 [3]沈阳海关技术中心 [4]大连海关技术中心

出　　处：《中国果树》2024年第7期81-88,共8页China Fruits

基　　金：宁夏回族自治区教育厅高等学校科学研究项目(NYG2022232);海关总署科研项目(2023HK083);大连海关科研项目(2023DK01);“十四五”国家重点研发计划课题(2021YFD1400100)。

摘　　要：葡萄白粉病是葡萄栽培中最重要的真菌病害之一,其病原物为专性寄生活体营养型葡萄白粉菌(Erysiphe necator)。分析了TUB2基因是否可以作为葡萄白粉菌及其近缘种菌株的DNA条形码,结果显示,TUB2基因在白粉菌属上具有明显的种间和种内遗传变异间距(Barcoding Gap),系统树分析显示TUB2基因对葡萄白粉菌及其近缘种具有有效的区分能力,因此认为TUB2基因可作为葡萄白粉病菌诊断的理想DNA条形码候选片段。Grape powdery mildew is one of the most important fungal diseases in viticulture,and its pathogen is Erysiphe necator.In this study,we investigated whether the TUB2 gene could serve as a DNA barcoding differentiating Erysiphe necator and its closely-related species.The results revealed that the TUB2 genes show distinct discrepancies between intra-and inter-specific genetic distances in Erysiphe sepeices.The diversity of the TUB2 genes can effectively distinguish Erysiphe necator and its closely-related species via phylogenetic study.Therefore,the TUB2 region could be recommended as the ideal candidate DNA barcoding for the diagnose of grape powdery mildew.

关 键 词：TUB2 葡萄白粉菌 DNA条形码 生物信息学 

分 类 号：S436.631.19[农业科学—农业昆虫与害虫防治]