铽-金属有机骨架对色氨酸的荧光识别与检测  

Fluorescence identification and detection of tryptophan by terbium-metal organic framework

作　　者：尚可霞 王国云 邵元萍 张文超 何文涛 SHANG Ke-xia;WANG Guo-yun;SHAO Yuan-ping;ZHANG Wen-chao;HE Wen-tao(Medical College,Hexi University,Zhangye 734000,China;Gansu Provincial Key Laboratory of Characteristic Resources Utilization in Hexi Corridor,Zhangye 734000,China)

机构地区：[1]河西学院医学院,甘肃张掖734000 [2]甘肃省河西走廊特色资源利用重点实验室,甘肃张掖734000

出　　处：《现代化工》2024年第8期232-237,共6页Modern Chemical Industry

基　　金：甘肃省高校教师创新基金项目(2023B-178,2024B-158);国家大学生创新创业训练计划项目(202210740062);河西学院博士科研启动基金(KYQD2020014)。

摘　　要：采用水热法制备了铽-金属有机骨架(Tb-MOF),并用于常见氨基酸的识别和检测。探究了pH、有机溶剂、干扰氨基酸对Tb-MOF荧光强度的影响。结果表明,该固态Tb-MOF作为荧光传感器可在水溶液中稳定存在,具有优良的pH稳定性、溶剂稳定性和发光稳定性。荧光传感测试结果表明,在所研究的13种常见氨基酸中,色氨酸能使Tb-MOF的荧光信号发生荧光猝灭且猝灭效果最佳,猝灭百分数达79.0%,比荧光猝灭效果次之的甲硫氨酸(猝灭百分数为48.0%)高出30%以上。色氨酸的荧光猝灭系数K_(sv)=4.73×103 M^(-1)。Tb-MOF对色氨酸的检出限为0.1008μmoL/L,可抵御多种氨基酸的干扰。Tb-MOF可以通过肉眼实现色氨酸的有效识别,且对色氨酸的检测具有低的检测限和良好的抗干扰能力。A terbium-metal organic framework(Tb-MOF)is prepared via hydrothermal method,and applied for the recognition and detection of common amino acids.The effects of pH,organic solvent and interfering amino acids on the fluorescence intensity of Tb-MOF are investigated.It is shown that this solid Tb-MOF can stably exist in aqueous solution,and has excellent pH stability,solvent stability and luminescence stability.It is indicated from the fluorescence sensing test results that tryptophan can quench the fluorescence signal of Tb-MOF,and deliver the best quenching effect among thirteen kinds of common amino acids studied,with a quenching percentage of 79.0%,which is more than 30%higher than methionine(48.0%)that has the second fluorescence quenching effect among amino acids studied.The fluorescence quenching coefficient of tryptophan K_(sv) is 4.73×10^(3) M^(-1).The detection limit of Tb-MOF for tryptophan is 0.1008μmoL·L^(-1),which can resist the interference of various other amino acids.Tryptophan can be recognized effectively by the naked eye by means of Tb-MOF,and the detection of tryptophan by Tb-MOF has low detection limit and good anti-interference ability.

关 键 词：铽-金属有机骨架 色氨酸 荧光识别 检测 

分 类 号：O62[理学—有机化学]