年轻和老年小鼠脂肪来源干细胞生物学特性对比  

Comparison of biological characteristics of adipose-derived stem cells in young and old mice

作　　者：林美玉 姚翔 高景 赵玺龙 潘兴华 阮光萍 Lin Meiyu;Yao Xiang;Gao Jing;Zhao Xilong;Pan Xinghua;Ruan Guangping(Basic Medical Laboratory of 920 Hospital of the Joint Logistics Support Force of Chinese PLA,Kunming 650032,Yunnan Province,China;Integrated Engineering Research Center of Cell Biological Medicine of State and Regions,Kunming 650032,Yunnan Province,China;Transfer Medicine Key Laboratory of Cell Therapy Technology of Yunan Province,Kunming 650032,Yunnan Province,China)

机构地区：[1]解放军联勤保障部队第九二〇医院基础医学实验室,云南省昆明市650032 [2]干细胞与免疫细胞生物医药技术国家地方联合工程研究中心,云南省昆明市650032 [3]云南省细胞治疗技术转化医学重点实验室,云南省昆明市650032

出　　处：《中国组织工程研究》2025年第19期4063-4068,共6页Chinese Journal of Tissue Engineering Research

基　　金：云南省科技计划项目重大科技专项(2018ZF007),项目负责人:潘兴华;云南省重点项目(202101AS070039),项目负责人:阮光萍;中国人民解放军联勤保障部队第九二〇医院院管课题(2020YGD12),项目负责人:阮光萍。

摘　　要：背景:脂肪来源干细胞具有抗衰老的作用,但来自不同年龄供者的脂肪来源干细胞是否有区别,还需深入研究。目的:对比老年和年轻小鼠脂肪来源干细胞的生物学特性。方法:分别从8周龄、14周龄C57BL小鼠脂肪组织中提取脂肪来源干细胞,对比分析老年和年轻小鼠脂肪来源干细胞的细胞周期、凋亡、增殖能力的差异,定量PCR和Western blot检测老年和年轻小鼠脂肪来源干细胞衰老相关P21、P27基因和蛋白的表达。结果与结论:与老年小鼠脂肪来源干细胞相比,年轻小鼠脂肪来源干细胞更有活力,形态更规则,凋亡更少,增殖更快,衰老相关P21、P27基因和蛋白表达量更低,说明年轻小鼠脂肪来源干细胞具有更好的抗衰老作用。BACKGROUND:Adipose-derived stem cells have anti-aging effects,but whether adipose-derived stem cells from donors of different ages are different needs further study.OBJECTIVE:To compare the biological properties of adipose-derived stem cells in old and young mice.METHODS:Adipose-derived stem cells were extracted from adipose tissue of C57BL mice aged 8 and 14 weeks,respectively.The differences of cell cycle,apoptosis,and proliferation of adipose-derived stem cells in old and young mice were compared.The expression levels of aging-related P21 and P27 genes and proteins of adipose-derived stem cells in old and young mice were detected by quantitative PCR and western blot assay.RESULTS AND CONCLUSION:Compared with old mouse adipose-derived stem cells,young mouse adipose-derived stem cells were more active,more regular in morphology,less apoptosis,faster proliferation,and lower in expression of age-related P21 and P27 genes and proteins.It has been proven that adiposederived stem cells from young mice have better anti-aging effects.

关 键 词：脂肪来源干细胞 脂肪组织 年轻 老年 小鼠 抗衰老 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] Q2[生物学—细胞生物学]