响应曲面法优化茯苓多糖微波辅助提取工艺  

Optimisation of Microwave-Assisted Extraction of Poria Cocos Polysaccharides by Response Surface Methodology

作　　者：王进[1] 彭浩[1,2] 解修超 乔伟[3] 乔艳明 WANG Jin;PENG Hao;JIE Xiuchao;QIAO Wei;QIAO Yanming(School of Bioscience and Engineering,Shaanxi University of Technology,Hanzhong 723000,Shaanxi,China;Shaanxi Engineering Research Center of Edible and Medicated Fungi,Hanzhong 723000,Shaanxi,China;College of Life Sciences,Yulin University,Yulin 719000,Shaanxi,China)

机构地区：[1]陕西理工大学生物科学与工程学院,陕西汉中723000 [2]陕西省食药用菌工程技术研究中心,陕西汉中723000 [3]榆林学院生命科学学院,陕西榆林719000

出　　处：《安康学院学报》2024年第4期122-128,共7页Journal of Ankang University

基　　金：陕西省重点研发计划项目“大球盖菇种质资源引进、优选及配套栽培技术研究”(2020NY-062);陕西省教育厅专项项目“Si3N4增强30CrNi2MoV金属基梯度功能材料的制备及性能研究”(22JK0309);陕西理工大学校级科研项目“β型TiNbMoZr生物医用钛合金成分设计及腐蚀行为研究”(SLGKYXM2205)。

摘　　要：以茯苓菌核为原料,应用响应曲面法优化微波辅助提取茯苓多糖的工艺。在单因素实验基础上,通过Box-Behnken实验设计以及响应曲面分析法,研究料液比、pH值、微波功率、提取时间、提取次数对茯苓多糖提取率的影响。结果表明最佳提取工艺为:液料比28.3:1、pH6.8、微波功率548W、提取时间2.2min、提取次数2次。在此条件下,茯苓多糖提取率为3.04%,与预测值3.06%的相对误差小于5%。该法得到的提取工艺参数可靠,可为茯苓多糖工业化生产提供技术参考。Response surface method was used to optimize microwaves assisted extraction of polysaccharide from Poria sclerotium.On the basis of single factor experiment,Box-Behnken experiment design and response surface analysis were used to study the effects of solid-liquid ratio,pH value,microwave power,extraction time and extraction times on the extraction yield of Poria polysaccharide.The results showed that the optimal extraction process was as follows:liquid-solid ratio 28.3:1,pH6.8,microwave power 548W,extraction time 2.2min,extraction times 2 times.Under these conditions,the extraction yield was 3.04%,and the relative error between the extraction yield and the predicted value was 3.06%was less than 5%.The extraction parameters obtained by this method are reliable and can provide technical reference for the industrial production of poria polysaccharide.

关 键 词：茯苓 多糖 微波辅助 BOX-BEHNKEN设计 响应曲面法 

分 类 号：R284.2[医药卫生—中药学]