罂粟种质资源遗传多样性和亲缘关系的SRAP分析  

SRAP Analysis of the Genetic Diversity and Genetic Relationship of Poppy Germplasm Resources

作　　者：王玉红 王笑 张兆萍 魏玉杰 苏毓杰 杨振华 WANG Yu-hong;WANG Xiao;ZHANG Zhao-ping;WEI Yu-jie;SU Yu-jie;YANG Zhen-hua(Gansu Academy of Agri-Engineering Technology,Lanzhou,Gansu 730000,China;Key Laboratory of the Special Medicine Source Plant for Germplasm Innovation and Safety Utilization,Wuwei,Gansu 733006,China;Hexi Comprehensive Experimental Station of National Traditional Chinese Medicine Industry Technology System,Wuwei,Gansu 733006,China)

机构地区：[1]甘肃省农业工程技术研究院,甘肃兰州730000 [2]甘肃省特种药源植物种质创新与安全利用重点实验室,甘肃武威733006 [3]国家中药材产业技术体系河西综合试验站,甘肃武威733006

出　　处：《福建农业科技》2024年第5期33-38,共6页Fujian Agricultural Science and Technology

基　　金：甘肃省青年科技基金计划项目(21JR7RA748);国家现代农业产业技术体系(CARS-21)。

摘　　要：探明罂粟种质资源遗传多样性和亲缘关系,为罂粟遗传育种研究和种质指纹图谱奠定工作基础。利用形态标记和SRAP分子标记两种方法对25份罂粟资源的13个农艺性状进行测定、分析。结果表明:从12对SRAP引物中共筛选出11对多态性明显、条带清晰的引物组合,对25份供试材料基因组DNA分子进行扩增,共扩增出210条谱带,其中多态性条带81条,多态性比率为38.57%。SRAP聚类分析结果表明:25份罂粟材料的遗传相似系数在0.907~0.992,当相似系数在0.936处作切割线,可将25份供试材料分为5个大类和若干亚类。对供试罂粟材料的花色、叶形等农艺性状进行调查分析,当欧式距离为5.0时,可将所有罂粟材料可以分为12个大类和若干亚类。综上分析表明25份罂粟种质资源遗传多样性较丰富,形态标记和SRAP分子聚类可以用于研究罂粟种质资源遗传多样性和亲缘关系分析,研究结果可为罂粟遗传育种研究和种质指纹图谱奠定工作基础。The genetic diversity and genetic relationship of poppy germplasm resources were explored,which would lay a foundation for genetic breeding research and germplasm fingerprint of poppy.The 13 agronomic traits of 25 poppy resources were measured and analyzed by using the two methods of morphological markers and SRAP molecular markers.The results showed that 11 pairs of primer combinations with obvious polymorphism and clear bands were selected from 12 pairs of SRAP primers.The genomic DNA molecules of the 25 tested materials were amplified,and a total of 210 spectrum bands were amplified,of which 81 were polymorphic bands,and the polymorphism rate was 38.57%.The results of SRAP cluster analysis showed that:the genetic similarity coefficient of 25 poppy materials was 0.907-0.992.The cutting line was made when the similarity coefficient was 0.936,and the 25 tested materials could be divided into 5 categories and several subcategories.The agronomic traits such as flower color and leaf shape of the tested poppy materials were investigated and analyzed.When the euclidean distance was 5.0,all the poppy materials could be divided into 12 categories and several subcategories.In summary,the analysis showed that the genetic diversity of 25 poppy germplasm resources was abundant.The morphological markers and SRAP molecular clustering could be used to study the genetic diversity and genetic relationship of poppy germplasm resources.The results could lay a foundation for the genetic breeding research and germplasm fingerprint of poppy.

关 键 词：罂粟 农艺性状 遗传多样性 SRAP标记 

分 类 号：S567.21[农业科学—中草药栽培]