饲粮添加角鲨烯对脂多糖刺激仔猪生长性能、代谢器官功能及抗氧化能力的影响  

Effects of dietary squalene supplementation alleviates LPS-induced growth performance,metabolic organs function and antioxidant capacity in a piglet model

作　　者：马雪 周应卿 杜鑫 张影梅 王涤非 叶枝旺 高阳 MA Xue;ZHOU Yingqing;DU Xin;ZHANG Yingmei;WANG Difei;YE Zhiwang;GAO Yang(College of Life Science,Baicheng Normal University,Baicheng,Jilin Province 137000,China;College of Animal Science and Technology,Jilin Agricultural University,Changchun,Jilin Province 130118,China;College of Animal Science and Technology,Yangzhou University,Yangzhou,Jiangsu Province 225009,China)

机构地区：[1]白城师范学院生命科学学院,吉林白城137000 [2]吉林农业大学动物科学技术学院,吉林长春130118 [3]扬州大学动物科学与技术学院,江苏扬州225009

出　　处：《中国饲料》2024年第15期77-84,共8页China Feed

基　　金：吉林省科技厅项目(JJKH20190017KJ);白城师范学院博士科研启动资金项目(012006);2023年大学生创新创业训练计划项目(202310206043)。

摘　　要：为研究饲粮添加角鲨烯(SQ)对脂多糖(LPS)刺激仔猪生长性能、代谢器官功能及抗氧化能力的影响,试验选取30头35日龄健康“杜×长×大”三元杂交仔猪,体重为(9.04±0.33)kg,均为去势雄性。将仔猪随机分为3个组(CON、LPS和SQL组),每组5个重复,每个重复2头猪。CON组和LPS组饲喂基础日粮,SQL组在基础日粮中添加300 mg/kg的SQ。预饲期7 d,正饲期10 d。在试验期第5天和8天,LPS组和SQL组腹膜注射100μg/kg BW的LPS,CON组注射等量的生理盐水。试验过程中记录仔猪采食量,第5天开始每日记录仔猪的腹泻和咳喘情况,试验结束后称重、屠宰全部仔猪,收集血液、肝脏和肾脏样本,用于测定肝肾功能、免疫和抗氧化等指标。结果表明:(1)与LPS组相比,SQL组仔猪的末重、平均日采食量(ADFI)和平均日增重(ADG)分别提高5.56%、13.64%和32%,差异显著(P<0.05),腹泻指数和咳喘指数分别降低27.59%和32.81%,差异显著(P<0.05),且与CON组差异不显著(P>0.05)。(2)与LPS组相比,CON组仔猪肝脏指数和肾脏指数分别降低10.33%和12%,SQL组仔猪肝脏指数和肾脏指数分别降低6.36%和6.61%,差异显著(P<0.05)。CON组仔猪血清肝功指标中谷丙转氨酶(ALT)、谷草转氨酶(AST)和胆红素水平相较于LPS组分别降低了17.31%、20.15%和48.05%,SQL组仔猪血清肝功指标中ALT、AST和胆红素水平相较于LPS组分别降低了12.03%、11.56%和11.69%,差异显著(P<0.05)。CON组和SQL组仔猪肾功指标中尿素氮(BUN)水平相较于LPS组分别降低了27.39%和14.64%,差异显著(P<0.05)。(3)与LPS组相比,CON组仔猪血清中超氧化物歧化酶(SOD)和总抗氧化力(TAC)分别提高29.45%和39.5%,肝脏中谷胱甘肽过氧化物酶(GSH-Px)和TAC分别提高65.94%和66.17%,肾脏中TAC提高43.41%,差异均显著(P<0.05)。SQL组仔猪血清中SOD和TAC分别提高22.02%和35.61%,肝脏中GSH-Px和TAC分别提高47.85%和50.75%,肾脏中TAC提高31.34%,差异均显著(P<0.05)。CON组仔猪血�The purpose of the experiment was to study the effects of supplementing dietary squalene(SQ)on the growth performance,metabolic organ,and antioxidant capacity which were stimulated by lipopolysaccharide(LPS)in a piglet model.Thirty 35-day-old castrated male commercial piglets with similar body weights were randomly divided into three groups(CON,LPS,SQL groups)with five replicates in each group and two piglets in each replicate.The CON and LPS groups were fed a basic diet,and the SQL group was fed a basal diet with 300 mg/kg SQ.The pre-feeding period was 7 days and the formal feeding period was 10 days.On the 5th and 8th day of the test,the LPS and SQL group were intraperitoneally injected with 100μg/kg BW LPS,the CON group was injected with the same amount of normal saline.During the test,the feed intake was recorded,and the diarrhea index and breathing conditions were recorded daily from the fifth day.After the test,all piglets were weighed and slaughtered,and the blood,liver,and kidney samples were collected to detect organ function,immune and antioxidant indexes.The results showed that compared with the LPS group,the final weight,average daily feed intake(ADFI),and average daily gain(ADG)of piglets in the SQL group increased by 5.56%,13.64%and 32%,respectively,with significant differences(P<0.05).The diarrhea index and cough and asthma index decreased by 27.59%and 32.81%,respectively,with significant differences(P<0.05),and there was no significant difference(P>0.05)compared with the CON group.Compared with the LPS group,the liver index and kidney index of piglets in the CON group decreased by 10.33%and 12%,respectively.The liver index and kidney index of piglets in the SQL group decreased by 6.36%and 6.61%,respectively,with significant differences(P<0.05).The levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),and bilirubin in the serum liver function indicators of piglets in the CON group decreased by 17.31%,20.15%and 48.05%compared to the LPS group,respectively.The levels of ALT,AST,and

关 键 词：角鲨烯 脂多糖 仔猪 生长性能 代谢器官功能 抗氧化 

分 类 号：S816.7[农业科学—饲料科学]