Fine-mapping and primary analysis of candidate genes associated with seed coat color in mung bean(Vigna radiata L.)  

作　　者：Qian Wang Huimin Cao Jingcheng Wang Zirong Gu Qiuyun Lin Zeyan Zhang Xueying Zhao Wei Gao Huijun Zhu Hubin Yan Jianjun Yan Qingting Hao Yaowen Zhang 

机构地区：[1]College of Agronomy,Shanxi Agricultural University,Taiyuan 030031,China 2 [2]Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China

出　　处：《Journal of Integrative Agriculture》2024年第8期2571-2588,共18页农业科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(32301928);the Basic Research Program of Shanxi Province,China(20210302124504);the China Agriculture Research System of MOF and MARA-Food Legumes(CARS08-G10);the National Laboratory Project of Coarse Grain Germplasm Resources Innovation and Molecular Breeding,China(K462202040-01);the Ph D of Shanxi Agricultural University Scientific Research Start-up Project,China(2021BQ43);the Scientific Research Project of Shanxi Agricultural University,China(YZGC098)。

摘　　要：Seed coat color affects the appearance and commodity quality of mung beans(Vigna radiata L.).The substances that affect mung bean seed coat color are mainly flavonoids,which have important medicinal value.Mapping the seed coat color gene in mung beans would facilitate the development of new varieties and improve their value.In this study,an F2 mapping population consisting of 546 plants was constructed using Jilv9(black seed coat)and BIS9805(green seed coat).Using bulk segregated analysis(BSA)sequencing and kompetitive allele-specific PCR(KASP)markers,the candidate region related to seed coat color was finally narrowed to 0.66 Mb on chromosome(Chr.)4 and included eight candidate genes.Combined transcriptome and metabolome analyses showed that three of the eight candidate genes(LOC106758748,LOC106758747,and LOC106759075)were differentially expressed,which may have caused the differences in flavonoid metabolite content between Jilv9 and BIS9805.These findings can provide a research basis for cloning the genes related to seed coat color and accelerate molecular markerassisted selection breeding in mung beans.

关 键 词：mung bean seed coat color bulk segregated analysis sequencing TRANSCRIPTOME METABOLISM FLAVONOIDS 

分 类 号：S522[农业科学—作物学]