Gga-miRNA-181-5p family facilitates chicken myogenesis via targeting TGFBR1 to block TGF-βsignaling  

作　　者：Xiaoxu Shen Yongtong Tian Wentao He Can He Shunshun Han Yao Han Lu Xia Bo Tan Menggen Ma Houyang Kang Jie Yu Qing Zhu Huadong Yin 

机构地区：[1]Key Laboratory of Livestock and Poultry Multi-omics,Ministry of Agriculture and Rural Affairs,College of Animal Science and Technology,Sichuan Agricultural University,Chengdu 611130,China [2]Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province,Sichuan Agricultural University,Chengdu 611130,China [3]College of Forestry,Sichuan Agricultural University,Chengdu 611130,China [4]College of Resources,Sichuan Agricultural University,Chengdu 611130,China [5]Triticeae Research Institute,Sichuan Agricultural University,Chengdu 611130,China [6]Key Laboratory for Animal Disease Resistance Nutrition of China,Institute of Animal Nutrition,Ministry of Education/Sichuan Agricultural University,Chengdu 611130,China

出　　处：《Journal of Integrative Agriculture》2024年第8期2764-2777,共14页农业科学学报（英文版）

基　　金：supported by the National Key Research and Development Program of China(2022YFF10002020);Sichuan Science and Technology Program,China(2021YFYZ0007 and 2021YFYZ0031).

摘　　要：MicroRNAs(miRNAs)have been demonstrated to control chicken skeletal muscle growth,however,the potential function of the miR-181-5p family in chicken myogenesis remains largely unknown.Here,our study identified the two chicken(Gallus gallus;Gga)miR-181-5p family members widely expressed in various tissues,specifically miR-181a-5p and miR-181b-5p.Besides,the breast muscles of fast-growing broilers expressed higher levels of miR-181a-5p and miR-181b-5p than those of slow-growing layers.Functionally,miR-181a-5p and miR-181b-5p both promote the expression level of myogenic factors including myogenin(MyoG),myogenic differentiation 1(MyoD1),and myosin heavy chain(MyHC),meanwhile accelerating the myotube formation of skeletal muscle satellite cells(SMSCs).Mechanistically,miR-181a-5p and miR-181b-5p directly bind to the 3′untranslated region(UTR)of the transforming growth factor beta receptor 1(TGFBR1)mRNA,further reducing the expression of TGFBR1.TGFBR1 is a key Transforming growth factor beta(TGF-β)signaling transduction receptor and had a negative function in muscle cell differentiation.Furthermore,knockdown of TGFBR1 facilitated the expression of chicken myogenic factors,boosted myotube formation,and decreased the SMAD family member 2/3(SMAD2/3)phosphorylation in chicken SMSCs.SMAD2/3 are downstream of TGF-βsignaling,and miR-181a-5p and miR-181b-5p could reduce the expression of TGFBR1 to further diminish the SMAD2/3 phosphorylation.Our findings revealed that the miR-181-5p family targets TGFBR1 to break the TGF-βsignaling transduction,which resulted in promoting chicken skeletal muscle development.

关 键 词：miRNA-181-5p family SMSCs differentiation TGFBR1 TGF-βsignaling 

分 类 号：S831.2[农业科学—畜牧学]