A CRISPR/Cas12a-based platform for rapid on-site bovine viral diarrhea virus diagnostics  

作　　者：Meixi Wang Jitao Chang Yuxin Han Chaonan Wang Songkang Qin Jun Wang Lulu Zhang Yuanmao Zhu Fei Xue Fang Wang Hongliang Chai Yulong Wang Xinjie Wang Xin Yin 

机构地区：[1]State Key Laboratory for Animal Disease Control and Prevention,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150069,China [2]Shenzhen Branch,Guangdong Laboratory of Lingnan Modern Agriculture,Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518000,China [3]College of Wildlife and Protected Area,Northeast Forestry University,Harbin 150040,China [4]Institute of Western Agriculture,Chinese Academy of Agricultural Sciences,Changji 831100,China

出　　处：《Journal of Integrative Agriculture》2024年第8期2872-2876,共5页农业科学学报（英文版）

基　　金：the Inner Mongolia Autonomous Region Major Science and Technology Project,China(2020ZD0006 and 2021ZD0013);the Natural Science Foundation of Heilongjiang Province,China(JQ2021C005);the National Key Research and Development Plan of China(2022YFE0140700);the National Natural Science Foundation of China(32172940)。

摘　　要：Bovine viral diarrhea virus(BVDV)is a positive-sense single-stranded RNA virus,belonging to the genus Pestivirus in the Flaviviridae family(Riitho et al.2020).Currently,BVDV is divided into 3 main genotypes,BVDV-1,BVDV-2,and BVDV-3,based on the genetic differences in the 5′untranslated region(5′UTR)(Muasya et al.2022).

关 键 词：BVDV CRISPR/Cas DIARRHEA 

分 类 号：S858.23[农业科学—临床兽医学]