蓝色七彩神仙鱼虹彩细胞呈色相关基因的发掘  

Exploration of candidate genes related to iridophore presenting blue skin in discus fish(Symphysodon haraldi)

作　　者：李中军 温彬 马腾飞[1,2,3] 刘鑫 高建忠[1,2,3,4] 陈再忠[1,2,3,4] LI Zhongjun;WEN Bin;MA Tengfei;LIU Xin;GAO Jianzhong;CHEN Zaizhong(Key Laboratory of Freshwater Aquatic Genetic Resources,Ministry of Agriculture and Rural Affairs,Shanghai Ocean University,Shanghai 201306,China;Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Ministry of Education,Shanghai Ocean University,Shanghai 201306,China;Shanghai Engineering Research Center of Aquaculture,Shanghai Ocean University,Shanghai 201306,China;National Demonstration Centre for Experimental Fisheries Science Education,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海海洋大学,农业农村部淡水水产种质资源重点实验室,上海201306 [2]上海海洋大学,水产种质资源发掘与利用教育部重点实验室,上海201306 [3]上海海洋大学,上海水产养殖工程技术研究中心,上海201306 [4]上海海洋大学,水产科学国家级实验教学示范中心,上海201306

出　　处：《水产学报》2024年第8期40-52,共13页Journal of Fisheries of China

基　　金：上海市自然科学基金(20ZR1423600);上海扬帆人才计划(19YF1419400)。

摘　　要：为发掘蓝色体色中与虹彩细胞呈色相关的基因,实验选取全蓝、白化全蓝七彩神仙鱼分别与全白七彩神仙鱼的皮肤组织,进行比较转录组学分析。结果显示,全蓝与全白七彩神仙鱼皮肤组织转录组序列比对,共有2192个差异表达基因(DEGs),其中1270个基因上调,922个基因下调,DEGs主要富集至嘌呤核苷酸生物合成过程、肌动蛋白结构组织、嘌呤化合物生物合成过程、氧化磷酸化和柠檬酸循环等代谢通路。白化全蓝与全白七彩神仙鱼转录组序列比对结果显示,共有3168个DEGs,其中1859个基因上调,1309个基因下调,主要富集在氧化磷酸化、核糖核苷二磷酸代谢过程、ADP代谢过程、阳离子结合和甘氨酸、丝氨酸和苏氨酸代谢等通路。为进一步发掘与虹彩细胞呈色相关的候选基因,通过数据库比对分析,在全蓝与全白七彩神仙鱼筛选出32个DEGs,在白化全蓝与全白七彩神仙鱼筛选出38个DEGs,其中alkal2b、gpnmb、fhl2、pka与虹彩细胞发育相关,pgam、prtfdc1、pnp、slc23a1、slc2a9、rab38、rdh10、psat1、paics与虹彩细胞中鸟嘌呤合成及运输相关。本研究为深入解析鱼类蓝色结构色的形成和调控机制奠定基础。The comparative transcriptomic analysis was performed on natural and albino blue discus with white discus fish(Symphysodon haraldi)respectively to excavate genes related to iridophores in blue skin.It was found a total of 2,192 different expression genes(DEGs)in natural blue fish,including 1,270 up-regulated and 922 downregulated genes.The DEGs were primarily enriched in purine nucleotide biosynthetic process,actomyosin structure organization,purine-containing compound biosynthetic process,oxidative phosphorylation,citrate cycle and other functions.For the albino blue discus fish compared to white one,there were 1,859 up-regulated and 1,309 down-regulated DEGs.Similarly,the dominant enriched pathways of these genes were oxidative phosphorylation,ribonucleoside diphosphate metabolic process,ADP metabolic process,cation binding,glycine,serine and threonine metabolism and other functions.In order to further explore candidate genes participating in iridophores appearing color,by comparing the database,a total of 32 and 38 DEGs were screened out in natural blue and albino blue discus,respectively.It could be predicted that alkal2b,gpnmb,fhl2 and pka were associated with the development of iridophores,and pgam,prtfdc1,pnp,slc23a1,slc2a9,rab38,rdh10,psat1,and paics might attend guanine synthesis and transportation in iridophores.This study might ground for in-depth analysis of the formation and regulation mechanism of blue structural color in fish.

关 键 词：鱼类 虹彩细胞 结构色 蓝色 

分 类 号：Q781[生物学—分子生物学] S965.8[农业科学—水产养殖]