斑褐孔菌多糖的分离纯化及结构表征  

Extraction,purification and structural analysis of polysaccharides from Fuscoporia punctata(Fr.)Cunn

作　　者：刘彤 申永叶 贾启超 郭远强[2] LIU Tong;SHEN Yongye;JIA Qichao;GUO Yuanqiang(College of Traditional Chinese Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China;College of Pharmacy,Nankai University,Tianjin 300350,China)

机构地区：[1]天津中医药大学中药学院,天津301617 [2]南开大学药学院,天津300350

出　　处：《药学研究》2024年第7期653-658,666,共7页Journal of Pharmaceutical Research

基　　金：国家自然科学基金项目(No.22377061)。

摘　　要：目的对从斑褐孔菌中分离纯化出的均一酸性多糖BHP90-3L进行结构解析和表征。方法采用DEAE-琼脂糖凝胶、Sephadex G-75等柱色谱,对斑褐孔菌多糖进行分离纯化。运用单糖组成分析、红外分析、甲基化分析和核磁共振图谱分析等方法,对分离得到的斑褐孔菌酸性多糖BHP90-3L的绝对分子量(M_(w))、单糖组成、糖苷键类型、糖苷键连接方式和构象等进行研究,确定其结构。结果BHP90-3L是一种分子量为9.489×10^(3) Da的酸性杂多糖,主要含有甘露糖、葡萄糖醛酸、葡萄糖、半乳糖,各单糖组成摩尔比为2.78∶1.72∶7.17∶1.00;由GC-MS和核磁结果推测BHP90-3L主链连接方式是→6)-α-D-Man p-(1→、→3)-β-D-Glc p-(1→、→3)-β-D-Glc p A-(1→、→3,4)-α-D-Man p-(1→、→6)-α-D-Man p-(1→,其中→3)-β-D-Glc p-(1→、→3)-β-D-Glc p A-(1→均具有重复单元,侧链仅有β-D-Gal p-(1→。刚果红试验表明BHP90-3L不具有三股螺旋结构。结论斑褐孔菌多糖BHP90-3L是从斑褐孔菌中分离得到的新的均一酸性多糖,对其结构的研究,为斑褐孔菌多糖的进一步开发利用提供参考依据。Objective To analyze and characterize the structure of BHP90-3L,a homogeneous acidic polysaccharide isolated and purified from Fuscoporia punctata(Fr.)Cunn.Methods Using DEAE Sepharose,Sephadex G-75,and other column chromatography techniques,the polysaccharides of F.punctata(Fr.)Cunn were isolated and purified.The absolute molecular weight(M_(w)),monosaccharide composition,type of glycosidic linkage,and conformation of the isolated acidic polysaccharide BHP90-3L from F.punctata(Fr.)Cunn were investigated to determine its structure through monosaccharide composition analysis,infrared analysis,methylation analysis,and NMR spectroscopy data analysis.Results BHP90-3L is an acidic heteropolysaccharide with a molecular weight of 9.489×10^(3) Da,mainly containing mannose,glucuronic acid,glucose,and galactose,with a molar ratio of 2.78∶1.72∶7.17∶1.00 for the monosaccharide composition.From the GC-MS and NMR results,it is hypothesized that the main chain of BHP90-3L is composed of→6)-α-D-Man p-(1→,→3)-β-D-Glc p-(1→,→3)-β-D-Glc p A-(1→,→3,4)-α-D-Man p-(1→,→6)-α-D-Man p-(1→.Among them,→3)-β-D-Glc p-(1→and→3)-β-D-Glc p A-(1→)both have repeating units,and the side chain has only β-D-Gal p-(1→.The Congo red experiment indicates that BHP90-3L does not have a three-stranded helical structure.Conclusion BHP90-3L,a new homogeneous acidic polysaccharide,was isolated from F.punctata(Fr.)Cunn.The study of its structure provides a reference for the further development and utilization of F.punctata(Fr.)Cunn polysaccharides.

关 键 词：斑褐孔菌 酸性多糖 分离纯化 结构解析 

分 类 号：R284.2[医药卫生—中药学]