工业大麻LecRLK基因家族的全基因组水平筛选及生物信息学分析  

Genome-wide screening and bioinformatics analysis of Cannabis sativa LecRLK gene family

作　　者：李岚 宁康 蔡晓琪 谢安 徐敏 徐沛 LI Lan;NING Kang;CAI Xiao-qi;XIE An;XU Min;XU Pei(College of Life Sciences,China Jiliang University,Hangzhou 310018,China)

机构地区：[1]中国计量大学生命科学学院,浙江杭州310018

出　　处：《中国中药杂志》2024年第14期3749-3757,共9页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金青年科学基金项目(82204561)。

摘　　要：凝集素类受体蛋白激酶(lectin receptor-like kinase,LecRLK)是一类带有凝集素保守域的植物激酶,在植物抵抗生物胁迫和非生物胁迫过程中起着重要作用,同时也参与植物生长发育过程。工业大麻Cannabis sativa是一种重要的多用途植株,广泛应用于食品、纺织、医药等领域。对工业大麻LecRLK家族进行全基因组水平筛选以及表达分析,为工业大麻LecRLK家族功能分析提供科学参考。因此,该研究基于BLAST和HMM方法最终在工业大麻全基因组上共鉴定到93个LecRLKs,其中包括69个G型,23个L型和1个C型。随后对家族成员进行一系列生物信息学分析,初步揭示了工业大麻LecRLKs家族成员的蛋白理化性质,对家族成员启动子顺式作用元件预测结果显示家族成员受到激素和逆境响应的调控。表达分析则显示部分家族成员在根中有较高表达,可能参与抗逆过程;另一些成员在雌花中高表达,可能参与雌花发育。该研究为LecRLKs基因功能的深入研究提供一定的理论基础。同时通过表达分析筛选了可能参与工业大麻抗逆候选LecRLKs成员,为将来工业大麻抗逆品种筛选提供理论依据。Lectin receptor-like kinase(LecRLK)is a class of phytokinase with lectin conserved domain,which plays an important role in plant resistance to biological and abiotic stresses,as well as plant growth and development.Cannabis sativa is an important multi-purpose plant,widely used in food,textile,medicine,and other fields.Genome-wide screening and expression analysis of the LecRLK family of C.sativa were performed in this paper,so as to provide scientific reference for functional analysis of the LecRLK family of C.sativa.Based on BLAST and HMM methods,93 LecRLKs were identified in the whole genome of C.sativa,including 69 G types,23 L types,and one C types.Subsequently,a series of bioinformatics analyses were performed on the LecRLK family members,and the physicochemical properties of the protein of the LecRLK family members were initially revealed.The prediction of cis-acting elements of promoters in family members showed that family members were regulated by hormones and stress response.The expression analysis showed that some family members were highly expressed in the roots,which may participate in the process of stress resistance.Several members were highly expressed in female flowers and may be involved in female flower development.This study provides a theoretical basis for further study of LecRLK gene function.Meanwhile,the expression analysis screens candidate LecRLK members who may participate in the resistance of C.sativa,which provides a theoretical basis for the subsequent selection of C.sativa varieties against resistance.

关 键 词：工业大麻 凝集素类受体蛋白激酶 家族基因 发育 

分 类 号：S567.2[农业科学—中草药栽培]