槲皮素联合铁死亡抑制药对草酸钙诱导的HK-2细胞损伤的影响  

Effects of quercetin combined with iron death inhibitor on calcium oxalate-induced HK-2 cell damage

作　　者：聂丙飞[1] 陈煌辉 谭群杰 杜宇峰 NIE Bing-fei;CHEN Huang-hui;TAN Qun-jie;DU Yu-feng(Department of Urology,The First People's Hospital of Chenzhou City,Chenzhou 423000,HunanProvince,China;Department of Urology,The Second People's Hospital of Yizhang County,Chenzhou 423000,Hunan Province,China)

机构地区：[1]郴州市第一人民医院泌尿外科,湖南郴州423000 [2]宜章县第二人民医院泌尿外科,湖南郴州423000

出　　处：《中国临床药理学杂志》2024年第14期2028-2032,共5页The Chinese Journal of Clinical Pharmacology

摘　　要：目的探究槲皮素联合铁死亡抑制剂Ferrostain-1对草酸钙诱导的HK-2细胞损伤的影响。方法将HK-2细胞随机分为对照组(正常培养的细胞)、模型组(0.5 mmol·L^(-1)的草酸钙结晶)、槲皮素组(0.5 mmol·L^(-1)的草酸钙结晶+100μmol·L^(-1)槲皮素)、抑制剂组(0.5 mmol·L^(-1)的草酸钙结晶+8μmol·L^(-1) Ferrostain-1)、联合组(0.5 mmol·L^(-1)的草酸钙结晶+100μmol·L^(-1)槲皮素+8μmol·L^(-1) Ferrostain-1)。用细胞计数试剂盒8(CCK-8)实验检测细胞存活率,用蛋白质印迹实验检测细胞谷胱甘肽过氧化物酶(GPX4)等铁死亡相关蛋白表达,用流式细胞术和Tunel实验检测细胞凋亡,用试剂盒法检测细胞铁离子及抗氧化指标水平。结果对照组、模型组、槲皮素组、抑制剂组、联合组的细胞存活率分别为(100.00±2.55)%、(54.49±4.11)%、(64.26±6.30)%、(58.03±3.04)%和(79.37±4.29)%,GPX4蛋白表达水平分别为0.98±0.11、0.33±0.05、0.56±0.05、0.78±0.07和1.11±0.11,细胞凋亡率分别为(4.15±0.28)%、(23.12±2.49)%、(17.28±1.07)%、(15.08±1.41)%和(8.95±0.75)%,Fe2+水平分别为(100.00±0.87)%、(162.55±14.70)%、(149.09±9.50)%、(144.95±11.12)%和(131.76±12.18)%,超氧化物歧化酶(SOD)水平分别为(58.67±3.46)、(21.56±1.32)、(33.60±2.03)、(35.15±3.02)和(44.27±3.89)U·mL^(-1);模型组的上述指标与对照组比较,槲皮素组、抑制剂组、联合组的上述指标与模型组比较,联合组的上述指标与槲皮素组、抑制剂组比较,在统计学上差异均有统计学意义(均P<0.05)。结论铁死亡抑制剂可增强槲皮素体外对草酸钙诱导的肾小管上皮细胞损伤的抑制作用。Objective To explore the effect of quercetin combined with iron death inhibitor Ferrostain-1 on oxalate-induced HK-2 cell injury.Methods HK-2 cells were randomly divided into control group(normal cultured cells),model group(0.5 mmol·L^(-1)calcium oxalate crystals),quercetin group(0.5 mmol·L^(-1)calcium oxalate crystals+100μmol·L^(-1)quercetin),inhibitor group(0.5 mmol·L^(-1)calcium oxalate crystals+8μmol·L^(-1)Ferrostain-1)and combination group(0.5 mmol·L^(-1)calcium oxalate crystals+100μmol·L^(-1)quercetin+8μmol·L^(-1)Ferrostain-1).Cell counting kit-8(CCK-8)assay was used to detect cell survival rate;Western blot was used to detect iron death related protein expression such as glutathione peroxidase 4(GPX4);flow cytometry and Tunel assay were used to detect cell apoptosis,and assay kit was used to detect cellular iron ions and antioxidant levels.Results The cell survival rates of control group,model group,quercetin group,inhibitor group and combination group were(100.00±2.55)%,(54.49±4.11)%,(64.26±6.30)%,(58.03±3.04)%and(79.37±4.29)%,respectively;GPX4 protein expression levels were 0.98±0.11,0.33±0.05,0.56±0.05,0.78±0.07 and 1.11±0.11,respectively;cell apoptosis rates were(4.15±0.28)%,(23.12±2.49)%,(17.28±1.07)%,(15.08±1.41)%and(8.95±0.75)%,respectively;Fe^(2+)levels were(100.00±0.87)%,(162.55±14.70)%,(149.09±9.50)%,(144.95±11.12)%and(131.76±12.18)%,respectively;superoxide dismutase(SOD)levels were(58.67±3.46),(21.56±1.32),(33.60±2.03),(35.15±3.02)and(44.27±3.89)U·mL^(-1),respectively.The above indicators of the model group were compared with the control group,and the above indicators of the quercetin group,inhibitor group,and combination group were compared with the model group,the above indicators of the combination group were compared with the quercetin group,and inhibitor group,all they all showed statistical significance(all P<0.05).Conclusion Iron death inhibitors can enhance the inhibitory effect of quercetin in vitro on oxalate-induced renal tubular epithelial cell i

关 键 词：槲皮素 草酸钙肾结石 铁死亡 细胞损伤 细胞凋亡 

分 类 号：R28[医药卫生—中药学]