糖尿病小鼠来源的骨髓间充质干细胞培养上清液诱发胰岛素抵抗的研究  

Study on insulin resistance induced by supernatant of bone marrow mesenchymal stem cells derived from diabetic mice

作　　者：李包娟 周克春 阿布都拉·米热合买提 祖丽胡马·热合曼 叶雨萌 张之 LI Bao-juan;ZHOU Ke-chun;ABUDOULA·Mi-re-he-mai-ti;ZULIHUMA·Re-he-man;YE Yu-meng;ZHANG Yan-zhi(School of Pharmacy,Xinjiang Medical University,Xinjiang Medical University,Urumqi 830000,Xinjiang Uygur Autonomous Region,China;Key Laboratory of Active Components of Natural Medicine and Drug Release Technology,Xinjiang Medical University,Urumqi 830000,Xinjiang Uygur Autonomous Region,China)

机构地区：[1]新疆医科大学药学院,新疆维吾尔自治区乌鲁木齐830000 [2]新疆天然药物活性组分与释药技术重点实验室,新疆维吾尔自治区乌鲁木齐830000

出　　处：《中国临床药理学杂志》2024年第14期2033-2037,共5页The Chinese Journal of Clinical Pharmacology

基　　金：国家自然科学基金资助项目(81760767);新疆天然药物活性组分与释药技术重点实验室基金资助项目(XJDX1713)。

摘　　要：目的探究糖尿病小鼠来源的骨髓间充质干细胞及其旁分泌作用在诱发胰岛素抵抗(IR)中扮演的角色。方法建立小鼠糖尿病模型,提取并培养骨髓间充质干细胞(BMSC),收集培养上清液(M-BMSC-CS)。(1)细胞实验:将HepG2肝细胞分为正常低糖培养组[用低糖DMEM(5.55 mmol·L^(-1))进行培养]、M-BMSC-CS实验组(M-BMSC-CS 75μL)、高糖高脂对照组(给予25 mmol·L^(-1)高糖DMEM+0.25 mmol·L^(-1)棕榈酸)。(2)动物实验:小鼠分为正常小鼠组(腹腔注射等体积0.9%NaCl)和M-BMSC-CS-m组(M-BMSC-CS通过腹腔注射给予正常小鼠,注射剂量0.2 mL/10 g)。用葡萄糖氧化酶法检测葡萄糖摄取量,用免疫荧光法检测Glut2蛋白荧光强度,用蛋白质印迹法检测胰岛素信号通路蛋白表达;检测口服葡萄糖耐量(OGTT)和胰岛素耐量(ITT)。结果正常低糖培养组、M-BMSC-CS实验组和高糖高脂对照组的葡萄糖摄取量分别为(2.96±0.05)、(1.64±0.28)和(1.42±0.32)mmol·L^(-1),葡萄糖转运蛋白2(Glut2)荧光表达分别为53.21±2.70、30.95±3.39和34.96±7.60,磷酸化胰岛素受体底物1-ser307(p-IRS-1ser307)表达蛋白相对水平分别为0.46±0.21、1.09±0.24和0.91±0.16,磷酸化蛋白激酶(p-AKT)蛋白相对表达水平分别为0.94±0.05、0.59±0.06和0.53±0.05,Glut2蛋白表达水平分别为1.08±0.14、0.58±0.14和0.62±0.09;M-BMSC-CS实验组的上述指标与正常低糖培养组比较,在统计学上差异均有统计学意义(均P<0.05)。正常小鼠组和M-BMSC-CS-m组空腹血糖分别为(5.23±0.57)和(9.30±1.14)mmol·L^(-1),p-AKT蛋白相对表达水平分别为1.27±0.21和0.51±0.19,Glut2蛋白相对表达水平分别为1.17±0.17和0.79±0.09;M-BMSC-CS-m组的上述各指标与正常小鼠组比较,在统计学上差异均有统计学意义(均P<0.05)。结论糖尿病小鼠来源的BMSC培养上清液在体外引起正常HepG2肝细胞胰岛素抵抗,在体内诱发正常小鼠胰岛素抵抗。Objective To investigate the role of bone marrow mesenchymal stem cells derived from diabetic mice and their paracrine roles in inducing insulin resistance(IR).Methods The mouse model of diabetes mellitus was established,bone marrow mesenchymal stem cells(BMSC)were extracted and cultured,and the culture supernatant(M-BMSC-CS)was collected.(1)Cell experiment:HepG2 hepatocytes were divided into normal low-glycemic culture group[cultured with low-glycemic DMEM(5.55 mmol·L^(-1))],M-BMSC-CS experimental group(M-BMSC-CS75μL),and high-glycemic and high-lipid control group(given 25 mmol·L^(-1)high-glycemic DMEM+0.25 mmol·L^(-1)palmitic acid);(2)Animal experiments:Mice were divided into normal mice group(0.9%NaCl by intraperitoneal injection)and M-BMSC-CS-m group(M-BMSC-CS)by intraperitoneal injection of normal mice(injection dose0.2 mL/10 g).Glucose intake was measured by glucose oxidase method.The fluorescence intensity of Glut2 protein was detected by immunofluorescence.The expression of insulin signaling pathway protein was detected by Western blot.Test oral glucose tolerance(OGTT)and insulin tolerance(ITT).Results The glucose intakes of the normal low-glucose culture group,the M-BMSC-CS experimental group and the high-glucose and high-lipid control group were(2.96±0.05),(1.64±0.28)and(1.42±0.32)mmol·L^(-1),respectively;the fluorescence expressions of glucose transporter 2(Glut2)were 53.21±2.70,30.95±3.39 and 34.96±7.60,respectively;the protein expression levels of phosphorylated insulin receptor substrate 1-ser307(p-IRS^(-1)ser307)were 0.46±0.21,1.09±0.24 and 0.91±0.16,respectively;phosphorylated protein kinase(p-AKT)protein expression levels were0.94±0.05,0.59±0.06 and 0.53±0.05;Glut2 protein expression levels were 1.08±0.14,0.58±0.14 and0.62±0.09,respectively.The above indexes in M-BMSC-CS experimental group were statistically significant compared with those in normal low-glycemic culture group(all P<0.05).Fasting blood glucose levels in the normal group and M-BMSC-CS-m group were(5.23±0.57)and

关 键 词：糖尿病小鼠 骨髓间充质干细胞培养上清液 胰岛素抵抗 HepG2肝细胞 正常小鼠 

分 类 号：R97[医药卫生—药品]