橙皮苷促进小鼠急性皮肤创面愈合的作用研究  

Effects of hesperidin in promoting acute skin wound healing

作　　者：黄乙明 陈飞飞 张蓉蓉 朱光辉[1] HUANG Yi-ming;CHEN Fei-fei;ZHANG Rong-rong;ZHU Guang-hui(Department of Pharmacy,Yuying Children's Hospital,The Second Affiliated to Wenzhou Medical University,Wenzhou 325000,Zhejiang Province,China;Pharmacy Department,Taizhou Women and Children's Hospital of Wenzhou Medical University,Taizhou 318000,Zhejiang Province,China)

机构地区：[1]温州医科大学附属第二医院,育英儿童医院药学部,浙江温州325000 [2]温州医科大学附属台州妇女儿童医院药剂科,浙江台州318000

出　　处：《中国临床药理学杂志》2024年第14期2093-2097,共5页The Chinese Journal of Clinical Pharmacology

摘　　要：目的研究橙皮苷对人角质形成细胞(HaCaT)和人皮肤成纤维细胞(HSF)迁移能力的影响,初步探索橙皮苷促进小鼠急性皮肤创面愈合的作用及相关机制。方法将HaCaT和HSF分为空白组(不做任何处理)、对照组(加入0.1%二甲基亚砜)和实验组(加入5.0μg·mL^(-1)的橙皮苷),培养48 h。用细胞划痕实验检测细胞体外愈合能力,用Transwell迁移实验检测细胞迁移情况。将C57小鼠随机分为模型组和低、高剂量实验组,用手术剪剪下小鼠背部全层皮肤建立急性皮肤全层缺损创面模型。模型组给予0.5%二甲基亚砜,低、高剂量实验组分别给予10、50 mg·kg^(-1)橙皮苷溶液。用蛋白质印迹法检测β-连环蛋白、增殖细胞核抗原(PCNA)、角蛋白14、Ⅰ型胶原蛋白的表达情况。结果HaCaT-空白组、HaCaT-对照组和HaCaT-实验组的划痕愈合率分别为(21.05±1.10)%、(22.33±1.72)%、(41.61±2.90)%,细胞迁移数分别为(57.00±11.36)、(60.38±10.11)、(287.75±20.21)个;HSF-空白组、HSF-对照组和HSF-实验组的划痕愈合率分别为(17.82±1.62)%、(19.81±3.87)%、(64.22±1.94)%,细胞迁移数分别为(43.25±7.98)、(40.75±6.70)、(140.88±14.35)个。HaCaT-实验组的上述指标与HaCaT-空白组、HaCaT-对照组比较,HSF-实验组的上述指标与HSF-空白组、HSF-对照组比较,在统计学上差异均有统计学意义(均P<0.05)。模型组和低、高剂量实验组β-连环蛋白表达水平分别为0.53±0.06、0.74±0.17和1.44±0.11,角蛋白14相对表达水平分别为0.33±0.06、0.54±0.07和1.26±0.16,PCNA相对表达水平分别为0.46±0.05、0.72±0.09和1.14±0.11,Ⅰ型胶原蛋白相对表达水平分别为0.52±0.03、0.77±0.05和1.28±0.13。低、高剂量实验组的上述指标与模型组相比,在统计学上差异均有统计学意义(P<0.05,P<0.01)。结论橙皮苷可能通过激活Wnt/β-catenin信号通路,增加HaCaT和HSF的迁移,促进小鼠急性皮肤创面愈合。Objective To study the effects of hesperidin on the migration ability of human keratinocytes(HaCaT)and human skin fibroblasts(HSF).Additionally,this research aims to preliminary investigate the influence and underlying mechanism of Hesperidin in facilitating the healing process of acute skin wounds in mice.Methods HaCaT and HSF were divided into blank group(without any treatment),control group(added 0.1%dimethyl sulfoxide)and experimental group(added 5.0μg·mL^(-1)hesperidin)for 48 h.The healing ability of cells in vitro was detected by scratch test.The migration of cells was detected by Transwell migration test.C57 mice were randomly divided into model group,experimental-L,-H groups.The acute full-thickness skin defect wound model was established by surgical clipping of the full-thickness skin of the back of mice.The model group was given 0.5%dimethyl sulfoxide,and the experimental-L,-H groups were given 10,50 mg·kg^(-1)hesperidin solution,respectively.The protein expressions levels ofβ-catenin,proliferating cell nuclear antigen(PCNA),keratin 14 and collagenⅠwere detected by Western blot.Results The scratch healing rates of HaCaT-blank group,HaCaT-control group and HaCaT-experimental group were(21.05±1.10)%,(22.33±1.72)%and(41.61±2.90)%;the cell migration numbers were 57.00±11.36,60.38±10.11 and 287.75±20.21,respectively.The scratch healing rates of HSF-blank group,HSF-control group and HSF-experimental group were(17.82±1.62)%,(19.81±3.87)%and(64.22±1.94)%,the cell migration numbers were 43.25±7.98,40.75±6.70 and 140.88±14.35,respectively.The HaCaT-experimental group was compared with HaCaT-blank group and HaCaT-control group,and the HSF-experimental group was compared with HSF-blank group and HSF-control group,the differences were statistically significant(all P<0.05).The protein expression levels ofβ-catenin in the model group,experimental-L,-H groups were 0.53±0.06,0.74±0.17 and 1.44±0.11;the protein expression levels of keratin 14 were 0.33±0.06,0.54±0.07 and 1.26±0.16;the protein exp

关 键 词：橙皮苷 创面愈合 WNT/Β-CATENIN 人角质形成细胞 人皮肤成纤维细胞 

分 类 号：R28[医药卫生—中药学]