三黄洗剂指纹图谱建立及原药材相关性研究  

Establishment of high performance liquid chromatography fingerprint of Sanhuang Lotion and study on the correlation of original medicinal materials

作　　者：唐锦英 陈婷[1] 唐樑[1] 刘磊[1] 林梦 TANG Jinying;CHEN Ting;TANG Liang;LIU Lei;LIN Meng(Department of Pharmacy,Suzhou TCM Hospital Affiliated to Nanjing University of Chinese Medicine,Jiangsu Province,Suzhou215009,China)

机构地区：[1]南京中医药大学附属苏州市中医医院药学部,江苏苏州215009

出　　处：《中国当代医药》2024年第22期8-12,共5页China Modern Medicine

基　　金：江苏省苏州市科技发展计划项目(SYSD2019217)。

摘　　要：目的建立三黄洗剂高效液相色谱法(HPLC)指纹图谱,并分析与原药材的相关性,为该制剂的质量控制提供依据。方法采用ZORBAX Eclipse XDB-C18色谱柱(4.6 mm×250 mm,5μm),以甲醇-0.1%磷酸溶液为流动相(梯度洗脱),流速为1.0 ml/min,体积流量为1.0 ml/min,柱温为30℃,检测波长265 nm,进样量为10μl。结果建立起三黄洗剂的指纹图谱,共标定27个共有峰,指认出6个成分分别为黄芩苷、汉黄芩苷、黄芩素、小檗碱、芦荟大黄素和大黄酸,10批制剂的相似度均>0.95,与原药材的相关性良好。结论该方法简便、准确、重复性好,可以作为三黄洗剂质量控制的检测手段。Objective To establish the high performance liquid chromatography(HPLC)fingerprint of Sanhuang Lotion,and analyze the correlation of medicinal materials and finished products,to provide a basis for the quality control of the preparation.Methods The HPLC fingerprint analysis was performed on a 30℃ZORBAX Eclipse XDB-C18 chromatographic column(4.6 mm×250 mm,5μm),with the mobile phase consisting of methanol-0.1%phosphoric acid solution at the flow rate of 1.0 ml/min,the volume flow rate was 1.0 ml/min,the detection wavelength was set at 265 nm,and the injection volume was 10μl.Results The HPLC fingerprint of Sanhuang lotion was established,a total of 27 common peaks were calibrated.Six components were identified as baicalin,scutellarin,baicalein,berberine,aloe-emodin and rhein,and the similarities of the 10 batches of preparations were all>0.95.The correlation between medicinal materials and finished products was better.Conclusion The method is simple,accurate and reproducible,and can be used as a detection method for Sanhuang Lotion quality control.

关 键 词：三黄洗剂 指纹图谱 高效液相色谱法 聚类分析 

分 类 号：R28[医药卫生—中药学]