狼疮性肾炎患儿肾皮质及血清中干扰素-α/β表达水平及临床意义  

Expression levels and clinical significance of interferon-α/βin renal cortex and serum of children with lupus nephritis

作　　者：才桁 丁雪薇 陶思思 徐志泉 任翼[6] 向伟 张秋月 何小解[3] Cai Heng;Ding Xuewei;Tao Sisi;Xu Zhiquan;Ren Yi;Xiang Wei;Zhang Qiuyue;He Xiaojie(College of Pediatrics,Hainan Medical Cllege,Haikou 570312,China;Department of Pediatrics,Southern Medical University Southern Hospital,Guangzhou 510000,China;Institute of Pediatrics,The Second Xiangya Hospital,Central South University,Changsha 410011,China;Department of Health Management,Hospital of women and Children of Changsha,Changsha 410011,China;Hainan Provincial Women and Children's Medical center,Haikou 570312,China;Department of Pediatrics,Haikou Maternal and Child Health Center,Haikou 570203,China)

机构地区：[1]海南医学院儿科学院,海口570312 [2]南方医科大学南方医院儿科,广州510000 [3]中南大学湘雅二医院儿童医学中心,长沙410011 [4]长沙市妇幼保健院健康管理中心,长沙410011 [5]海南省妇女儿童医学中心,海口570312 [6]海口市妇幼保健院儿科,海口570203

出　　处：《中国医师杂志》2024年第7期1005-1009,1016,共6页Journal of Chinese Physician

基　　金：湖南省自然科学基金(2022JJ30064,2022JJ70048);湖南省临床医疗技术创新引导项目(2021SK53201);海南省自然科学基金(821RC1130,822RC871);海南省重点研发计划项目(ZDYF2021SHFZ088)。

摘　　要：目的分析狼疮性肾炎(LN)患儿肾皮质及血清中干扰素(IFN)-α/β表达水平及临床意义。方法选取2017年12月至2020年9月中南大学湘雅二医院儿童肾脏专科确诊的32例LN患儿为研究对象(LN组),正常肾脏对照来自3例正常肾移植志愿者活检肾组织(正常肾脏对照组),同时收集14例健康体检儿童作为正常对照组。依据系统性红斑狼疮疾病活动度指数(SLEDAI)将LN患儿分为轻度活动组(n=8),中度活动组(n=9)和重度活动组(n=15)。依据国际肾脏病学会/肾脏病理学会(ISN/RPS)2003LN分型标准进行病理分型(轻度病理损害组3例,中度病理损害组8例,重度病理损害组11例);采用免疫组织化学方法检测IFN-α/β在肾小球、肾间质中的表达与分布;采用酶联免疫吸附法检测血清样本中IFN-α/β浓度,分析其与LN患儿病理分型、病情活动的相关性。结果LN组血清、肾皮质IFN-α/β水平分别高于正常对照组与正常肾脏对照组(均P<0.05)。重度活动组血清IFN-α/β水平均高于轻、中度活动组(均P<0.05)。重度病理损害组血清、肾皮质IFN-α/β水平均高于轻、中度病理损害组(均P<0.05)。结论肾皮质中IFN-α/β与LN的肾损伤密切相关;血清IFN-α/β可在一定程度上辅助评估LN的疾病活动程度。Objective To analyze the expression levels and clinical significance of interferon(IFN)-cα/βin the renal cortex and serum of children with lupus nephritis(LN).Methods A total of 32 children with LN diagnosed in the pediatric nephrology department of the Second Xiangya Hospital of Central South University from December 2017 to September 2020 were selected as the study subjects(LN group).The normal kidney control group consisted of 3 normal kidney transplant volunteers who underwent biopsy of kidney tissue(normal kidney control group),while 14 healthy children who underwent physical examination were collected as the normal control group.According to the Systemic Lupus Erythematosus Disease Activity Index(SLEDAI),LN patients were divided into mild activity group(n=8),moderate activity group(n=9),and severe activity group(n=15).According to the International Society of Nephrology/Society of Nephrology(ISN/RPS)2003 LN classification criteria,pathological classification was performed(3 cases in the mild pathological damage group,8 cases in the moderate pathological damage group,and 11 cases in the severe pathological damage group);Immunohistochemistry was used to detect the expression and distribution of IFN-α/βin glomeruli and renal interstitium;Enzyme linked immunosorbent assay(ELISA)was used to detect the concentration of IFN-α/βin serum samples and analyze its correlation with the pathological classification and disease activity of LN patients.Results The serum and renal cortex IFN-α/βlevels in the LN group were higher than those in the normal control group and normal kidney control group,respectively(all P<0.05).The average level of serum IFN-α/βin the heavy activity group was higher than that in the light and moderate activity groups(all P<0.05).The serum and renal cortex IFN-α/βlevels in the severe pathological damage group were significantly higher than those in the mild and moderate pathological damage groups(all P<0.05).Conclusions IFN-α/βin the renal cortex is closely related to renal injury in

关 键 词：狼疮肾炎 干扰素Α 干扰素Β 

分 类 号：R725.9[医药卫生—儿科]