猪流行性腹泻病毒YL株分离鉴定及仔猪感染试验  

Isolation,Identification and Pathogenicity Testing of Porcine Epidemic Diarrhoea Virus YL Strain

作　　者：苏红萍 沈娜 麻文静 陈瑞 肖普辉 武永杰[4] 赵光明[5] 杨文欢[5] 吴艳 张磊 冯平[1] SU Hong-ping;SHEN Na;MA Wen-jing;CHEN Rui;XIAO Pu-hui;WU Yong-jie;ZHAO Guang-ming;YANG Wen-huan;WU Yan;ZHANG Lei;FENG Ping(College of Life Science,Yulin College,Yulin,Shaanri,719000,China;Shaanri Novelty Biotechnology Co.,Ltd,Yangling,Shaanzi,712100,China;Shaanzi Provincial Animal Husbandry Technology Extension General Station,Xi'an,Shaanri,710000,China;College of Veterinary Medicine,Northwest A&F University,Yangling,Shaanri,712100,China;Shaanri Provincial Infecious Disease Prevention and Control Center,Xi'an,Shaanri,710299,China)

机构地区：[1]榆林学院生命科学学院,陕西榆林719000 [2]陕西诺威利华生物科技有限公司,陕西咸阳712100 [3]陕西省畜牧技术推广总站,陕西西安710014 [4]西北农林科技大学动物医学院,陕西咸阳712199 [5]陕西省动物疫病预防控制中心,陕西西安710299

出　　处：《动物医学进展》2024年第8期120-124,共5页Progress In Veterinary Medicine

基　　金：陕西省教育厅服务地方专项项目(19JC043);榆林学院高层次人才科研启动基金项目(20GK12,20GK13);榆林市科技局产学研合作项目(2019-103-5)。

摘　　要：从腹泻仔猪肠道组织中分离获得猪流行性腹泻病毒(PEDV)地方分离株,并对其致病性进行初步分析。对陕西某养殖场采集有腹泻症状3~5日龄仔猪的十二指肠,经RT-PCR检测为PEDV核酸阳性。将阳性样品处理后接种Vero细胞进行病毒分离,并对分离毒株进行病毒含量测定、致病性试验。结果显示,分离到1株PEDV毒株(命名为YL株)。将分离的PEDV病毒液以不同滴度口服接种健康仔猪,2.0 mL/头,发现10^(5.5)TCID_(50)/mL剂量组接种1日后,100%(3/3)试验猪出现腹泻症状。To obtain local isolates of porcine epidemic diarrhoea virus(PEDV)from the intestinal tracts of piglets and to gain a preliminary understanding of their pathogenicity,in this study,the duodenum of piglets with diarrhoeal symptoms from 3 to 5 days of age was collected from a farm in Shaanxi,China,and the results of RT-PCR showed PEDV positive.The suspected positive samples were inoculated into Vero cells for virus isolation,and the isolated F5-generation viruses were subjected to RT-PCR detection,neutralisation test,viral content determination and pathogenicity test.The identification results showed that the isolated strain had the correct target gene and high viral content,and it was a specific strain against PEDV.We successfully isolated a strain of porcine epidemic diarrhoea virus(PEDV),named YL strain.The isolated PEDV virus solution was orally inoculated into healthy piglets at different levels,2.0 mL/head.It was found that 100%(3/3)of the test pigs showed diarrhoea symptoms 1 day after inoculation in the 105.5 TCIDso/mL dose group.The results of this study showed that the isolated strain was porcine epidemic diarrhoea virus(PEDV)with a minimum challenge dose of 10^(5.5)TCID_(50)/mL and 2.0 mL/head orally.The results of this test can provide theoretical reference for the isolation and identification of PEDV.

关 键 词：猪流行性腹泻病毒 分离 鉴定 最小攻毒剂量 

分 类 号：S852.659.6[农业科学—基础兽医学]