补充维生素D在桥本甲状腺炎动物模型中的作用及其机制研究  

The role and mechanism of vitamin D supplementation in an animal model of Hashimoto′s thyroiditis

作　　者：房方[1] 刘雅馨 冯文利 贾红蔚[1] 何庆[1] 朱梅[1] Fang Fang;Liu Yaxin;Feng Wenli;Jia Hongwei;He Qing;Zhu Mei(Department of Endocrinology and Metabolism,Tianjin Medical University General Hospital,NHC Key Laboratory of Hormones and Development,Tianjin Key Laboratory of Metabolic Diseases,Tianjin 300052,China)

机构地区：[1]天津医科大学总医院内分泌代谢科,国家卫生健康委员会激素与发育重点实验室,天津市代谢性疾病重点实验室,天津300052

出　　处：《中华内分泌代谢杂志》2024年第6期515-520,共6页Chinese Journal of Endocrinology and Metabolism

基　　金：天津市教委科研计划项目(2021KJ208)。

摘　　要：目的探究补充维生素D对桥本甲状腺炎(Hashimoto′s thyroiditis,HT)的影响及作用机制。方法选取雌性SD大鼠应用随机数字表法随机分为对照组、实验性自身免疫性甲状腺炎(experimental autoimmune thyroiditis,EAT)空白模型组(模型组)、小剂量(VD1组)和大剂量(VD2组)活性维生素D干预组。比较各组间甲状腺细胞形态、甲状腺功能、甲状腺抗体、各类CD4^(+)T细胞及相关细胞因子水平。结果模型组甲状腺过氧化物酶抗体(thyroid peroxidase antibody,TPOAb)和甲状腺球蛋白抗体(thyroglobulin antibody,TgAb)水平较对照组显著升高,VD1组和VD2组较模型组明显下降(P<0.05)。相比对照组,模型组HE染色示甲状腺组织内大量滤泡上皮细胞破坏,滤泡体积明显变小;相比模型组,VD1组和VD2组滤泡上皮细胞萎缩及破坏程度减轻。模型组辅助性T细胞(helper T cell,Th)1和Th17细胞比例及相关细胞因子水平显著高于对照组,而VD1组和VD2组均低于模型组(P<0.05);模型组调节性T细胞(regulatory T cell,Treg)比例及相关细胞因子水平显著低于对照组,而VD1组和VD2组均高于模型组(P<0.05)。结论补充维生素D后,EAT大鼠的TPOAb和TgAb水平下降,各类CD4^(+)T细胞数量及相关细胞因子水平均趋于正常,提示维生素D可能通过调节CD4^(+)T细胞的分化改善HT,从而为补充维生素D在治疗HT中的作用提供理论依据。ObjectiveTo investigate the effect and mechanism of vitamin D supplementation on Hashimoto′s thyroiditis(HT).MethodsFemale SD rats were randomly divided into four groups by random number table method:control group,experimental autoimmune thyroiditis(EAT)control model group(model group),low-dose(VD1 group)and high-dose(VD2 group)active vitamin D intervention groups.The morphology of thyroid cells,thyroid function,thyroid antibodies,various CD4^(+)T cells,and related cytokine levels among different groups were compared.ResultsThe levels of thyroid peroxidase antibody(TPOAb)and thyroid globulin antibody(TgAb)in model group were significantly higher than those in control group,while the levels of VD1 and VD2 groups were significantly lower than those in model group(P<0.05).Compared with control group,HE staining in model group showed severe damage of follicular epithelial cells;Compared with model group,the degree of atrophy and destruction of follicular epithelial cells in VD1 and VD2 groups were reduced.The proportion of helper T cell(Th)1 and Th17 cells and related cytokine levels in model group were significantly higher than those in control group,while those in VD1 and VD2 groups were lower than those in model group(P<0.05);The proportion of regulatory T cell(Treg)cells and related cytokine levels in model group were significantly lower than those in control group,while those in VD1 and VD2 groups were higher than those in model group(P<0.05).ConclusionsAfter supplementing with vitamin D,the levels of TPOAb and TgAb in EAT rats decreased,and the number of various CD4^(+)T cells and related cytokine levels tended towards normalization.This suggests that vitamin D may improve HT by regulating CD4^(+)T cell differentiation,providing a theoretical basis for the role of vitamin D supplementation in HT treatment.

关 键 词：桥本甲状腺炎 维生素D CD4^(+)T细胞 

分 类 号：R581.4[医药卫生—内分泌] R-332[医药卫生—内科学]