Evaluation of microRNA expression profiles in human sperm frozen using permeable cryoprotectant-free droplet vitrification and conventional methods  

作　　者：Li-Xin Zhang Jing Mao Yan-Dong Zhou Guang-Yao Mao Run-Fa Guo Hong-Shan Ge Xia Chen 

机构地区：[1]Department of Histology and Embryology,Medical School,Nantong University,Nantong 226001,China [2]Department of Reproductive Medicine,The Affiliated Taizhou People’s Hospital of Nanjing Medical University,Taizhou School of Clinical Medicine,Nanjing Medical University,Taizhou 225300,China.

出　　处：《Asian Journal of Andrology》2024年第4期366-376,共11页亚洲男性学杂志（英文版）

摘　　要：For sperm cryopreservation,the conventional method,which requires glycerol,has been used for a long time.In addition,the permeable cryoprotectant-free vitrification method has been continuously studied.Although the differences of cryopreservation effects between the two methods have being studied,differences in microRNA(miRNA)profiles between them remain unclear.In this study,we investigated the differences in miRNA expression profiles among conventional freezing sperm,droplet vitrification freezing sperm and fresh human sperm.We also analyzed the differences between these methods in terms of differentially expressed miRNAs(DEmiRs)related to early embryonic development and paternal epigenetics.Our results showed no significant differences between the cryopreservation methods in terms of sperm motility ratio,plasma membrane integrity,DNA integrity,mitochondrial membrane potential,acrosome integrity,and ultrastructural damage.However,sperm miRNA-sequencing showed differences between the two methods in terms of the numbers of DEmiRs(28 and 19 with vitrification using a nonpermeable cryoprotectant and the conventional method,respectively)in postthaw and fresh sperm specimens.DEmiRs related to early embryonic development and paternal epigenetics mainly included common DEmiRs between the groups.Our results showed that the differences between conventional freezing and droplet vitrification were minimal in terms of miRNA expression related to embryonic development and epigenetics.Changes in sperm miRNA expression due to freezing are not always detrimental to embryonic development.This study compared differences in miRNA expression profiles before and after cryopreservation between cryopreservation by conventional and vitrification methods.It offers a new perspective to evaluate various methods of sperm cryopreservation.

关 键 词：CRYOPRESERVATION cryoprotectant-free vitrification early embryonic development paternal epigenetic inheritance sperm miRNAs SUCROSE 

分 类 号：R698.2[医药卫生—泌尿科学]