‘红颜’草莓无糖组培生根技术研究及移栽基质筛选  

Research on Rooting Technology of‘Benihoppe’Strawberry Sugar-free Tissue Culture and Screening of Transplanting Substrate

作　　者：谢莹莹 黄敏 鲁峻宏 李晶 王立 宁眺 XIE Yingying;HUANG Min;LU Junhong;LI Jing;WANG Li;NING Tiao(School of Agriculture and Life Science,Kunming University/Engineering Research Center for Urban Modern Agriculture of Higher Education in Yunnan Province,Kunming,Yunnan 650214;College of Forestry,Southwest Forestry University,Kunming,Yunnan 650224;Shangri-la Zangmei Agricultural Technology Co.Ltd.,Diqing,Yunnan 674400;Jiangmen Xinhui Forestry Sciences Institute,Jiangmen,Guangdong 529199)

机构地区：[1]昆明学院农学与生命科学学院,云南省高校都市型现代农业工程中心,云南昆明650214 [2]西南林业大学林学院,云南昆明650224 [3]香格里拉市藏美农业科技有限公司,云南迪庆674400 [4]江门市新会林业科学研究所,广东江门529199

出　　处：《北方园艺》2024年第14期18-26,共9页Northern Horticulture

基　　金：春城计划青年拔尖人才资助项目(C201914005);云南省中青年学术与技术带头人资助项目(201914005)。

摘　　要：以‘红颜’草莓为试材,采用无糖组织培养方法,研究了‘红颜’草莓无糖组培生根技术,以期为草莓生产实践提供参考依据。结果表明:无糖组培最佳生根培养基为1/2MS+0.4 mg·L^(-1)IBA;传统组培最佳生根培养基为1/2MS+0.5 mg·L^(-1)IBA;培养条件以纯蛭石为基质,光照时长为12 h,CO_(2)通气时间在3 d时,草莓无糖组培幼苗生长最为健壮;在基质为草炭土∶蛭石∶珍珠岩=3∶1∶1配比下,草莓无糖组培苗移栽成活率达100%。Taking‘Benihoppe’strawberry as the test material,the rooting technology of‘Benihoppe’strawberry sugar-free tissue culture was studied by using the method of sugar-free tissue culture,in order to provide reference for strawberry production practice.The results showed that the best rooting medium for sugar-free tissue culture was 1/2MS+0.4 mg·L^(-1)IBA,the best rooting medium for traditional tissue culture was 1/2MS+0.5 mg·L^(-1)IBA,the strawberry sugar-free tissue culture seedlings grew most robustly when the substrate was pure vermiculite as the substrate,the light duration was 12 hours,and the CO_(2) ventilation time was 3 days,and the survival rate of strawberry sugar-free tissue culture seedlings was 100%under the ratio of peat-soil:vermiculite:perlite=3∶1∶1.

关 键 词：草莓 生根培养 无糖组培 移栽 

分 类 号：S668.4[农业科学—果树学]