响应面法优化两面针中三种生物碱的回流提取工艺  

Optimization of Reflux Extraction Process for Three Alkaloids From Zanthoxylum nitidum by Response Surface Method

作　　者：胡凤云[1] 乔丽娅 张占江[2] 董青松[2] 黄宝优[2] 朱艳霞[2] HU Fengyun;QIAO Liya;ZHANG Zhanjiang;DONG Qingsong;HUANG Baoyou;ZHU Yanxia(Guangxi Botanical Garden of Medicinal Plants/National Engineering Research Center of Southwest Endangered Medicinal Resource Development,Nanning,Guangxi 530023;Guangxi Botanical Garden of Medicinal Plants/National Center for Traditional Chinese Medicine Inheritance and Innovation,Nanning,Guangxi 530023)

机构地区：[1]广西壮族自治区药用植物园,西南濒危药材资源开发国家工程研究中心,广西南宁530023 [2]广西壮族自治区药用植物园,国家中医药传承创新中心,广西南宁530023

出　　处：《北方园艺》2024年第14期95-103,共9页Northern Horticulture

基　　金：广西科技计划资助项目(桂科AB22035068);广西中医药管理局广西特色药材关键技术研究推广资助项目(GZKJ2314-5)。

摘　　要：以两面针为试材,采用响应面法,研究了回流提取时间、提取溶剂浓度和料液比对氯化两面针碱、白屈菜红碱和血根碱提取得率的影响,以期获得两面针中3种生物碱的最佳回流提取工艺,为深入开发利用两面针中的生物碱提供参考依据。结果表明:3种生物碱的最优回流提取条件为提取时间2.4 h·次^(-1)(重复提取2次)、提取溶剂67%乙醇、料液比1∶20 g·mL^(-1),此时氯化两面针碱、白屈菜红碱和血根碱的得率分别为0.381%、0.560%、0.058%。该结果与响应面法预测值接近,说明该研究拟合的3种生物碱响应面回归模型可靠。优化后氯化两面针碱、白屈菜红碱和血根碱的得率分别提高了14%、95%、23%,优化工艺提取效率高,可用于两面针中3种生物碱的制备。Taking Zanthoxylum nitidum as the experimental material,the effects of reflux extraction time,extraction solvent concentration and solid-liquid ratio on the extraction rate of nitidine chloride,chelerythrine and sanguinarine were studied by response surface method,in order to obtain the optimal reflux extraction process of the three alkaloids,and provide reference for further development and utilization of alkaloids in Zanthoxylum nitidum.The results showed that the optimal reflux extraction condition of the three alkaloidsas follows,extraction time was 2.4 hours per time(repeated twice),extraction solvent was 67%ethanol,solid-liquid ratio was 1∶20 g·mL^(-1),and the yield of nitidine chloride,chelerythrine and sanguinarine were 0.381%,0.560%,0.058%,respectively.The results were closed to the predicted values of response surface method,it indicated that the three alkaloids response surface regression models fitted in this study were reliable.After optimization,the yield of nitidine chloride,chelerythrine and sanguinarine were increased by 14%,95%and 23%respectively,and the optimized extraction efficiency was high,which could be used for the preparation of three alkaloids in Zanthoxylum nitidum.

关 键 词：响应面法 两面针 回流提取 氯化两面针碱 白屈菜红碱 

分 类 号：S284.2[农业科学—农业水土工程]