大叶黄精转录组中SSR位点信息分析  

Analysis of SSR Site Information in the Transcriptome of Polygonatum kingianum var.grandifolium

作　　者：万小林 粱思琪 崔令军 肖强 WAN Xiaolin;LIANG Siqi;CUI Lingjun;XIAO Qiang(School of Forestry and Horticulture,Hubei Minzu University/Hubei Key Laboratory of Biologic Resources Protection and Utilization,Enshi,Hubei 445000)

机构地区：[1]湖北民族大学林学园艺学院,生物资源保护与利用湖北省重点实验室,湖北恩施445000

出　　处：《北方园艺》2024年第14期104-109,共6页Northern Horticulture

基　　金：国家自然科学基金资助项目(31260057);黄精黄酮合成分子机制解析与优质种质资源创新资助项目(2023AFD077);湖北省科技厅技术创新重大专项资助项目(2019ACA120)。

摘　　要：以大叶黄精转录组所获得的51704条Unigenes为试材,采用MISA软件检索出大叶黄精的SSR位点,并利用Primer 3软件对检索到的SSR基序进行引物设计,研究了大叶黄精的SSR特征,以期为大叶黄精SSR分子标记的开发与应用提供参考依据。结果表明:大叶黄精转录组中检索出25384个SSR位点,其发生频率为49.09%。单核苷酸和二核苷酸重复是大叶黄精SSR的主要基序类型,且A/T和AG/CT基序的出现频率最高,占大叶黄精SSR总数的66.91%。通过引物设计,最终在大叶黄精转录组中获得13220对引物。随机选取12对引物进行PCR扩增,扩增成功率高达67%。因此,认为该研究的转录组数据可靠,且SSR位点类型丰富。Taking 51704 Unigenes obtained from the transcriptome of P.kingianum var.grandifolium as the test material,and MISA software was used to retrieve the SSR loci of P.kingianum var.grandifolium and primers were designed for the retrieved SSR motifs using Primer 3 software,in order to provide reference for the development and application of SSR molecular markers in P.kingianum var.grandifolium.The results showed that 25384 SSR loci were retrieved from P.kingianum var.grandifolium transcriptome with a frequency of 49.09%.Single nucleotide and dinucleotide repeats were the major motif types of P.kingianum var.grandifolium SSR,and the A/T and AG/CT motifs had the highest frequency,accounting for 66.91%of the total number of P.kingianum var.grandifolium SSRs.By primer design,13220 primer pairs were finally obtained in P.kingianum var.grandifolium transcriptome.Twelve primer pairs were randomly selected for PCR amplification and the amplification success rate was high at 67%.Therefore,the transcriptome data from this study were considered reliable and rich in SSR locus types.

关 键 词：大叶黄精 转录组 SSR 

分 类 号：S668.9[农业科学—果树学]