铅中毒对大鼠肠道菌群和肠屏障功能的影响研究  

Effects of lead poisoning on intestinal microbe and barrier function in rats

作　　者：李硕[1,2] 王晶 李瑞仕[1] 韩耀巍[1] 刘菁[1] 杜洪喆[1] 陈祖明[1] 俎亚杰 李瑞本 李辉 LI Shuo;WANG Jing;LI Ruishi;HAN Yaowei;LIU Jing;DU Hongzhe;CHEN Zuming;ZU Yajie;LI Ruiben;LI Hui(Pediatric department,The First Affiliated Hospital of Tianjin University of Chinese Medicine,Tianjin 300381,China;不详)

机构地区：[1]天津中医药大学第一附属医院儿科,天津市300381 [2]国家中医针灸临床医学研究中心 [3]天津中医药大学第二附属医院 [4]武警陕西省总队汉中支队卫生队,陕西汉中723000

出　　处：《工业卫生与职业病》2024年第4期326-331,346,共7页Industrial Health and Occupational Diseases

基　　金：天津中医药大学第一附属医院“拓新工程”基金科研课题(院2020049)。

摘　　要：目的探究铅中毒对大鼠肠道菌群和肠屏障功能的影响,为防治铅中毒提供新的思路和方法。方法3周龄健康Wistar雄性大鼠随机分成正常组和模型组,每组6只,模型组每2日腹腔注射1次醋酸铅溶液(30 mg/kg体质量),共14 d。结束后,检测大鼠血清中D-乳酸和二胺氧化酶(diamine oxidase,DAO)水平;HE染色观察回肠组织形态;免疫组化染色观察闭合小环蛋白1(zonula occludens-1,ZO-1)含量;16 S rRNA测序检测回肠内容物中菌群相对丰度。结果铅中毒能改变大鼠肠道上皮细胞形态,降低紧密连接蛋白ZO-1水平,破坏肠黏膜屏障。铅中毒组血清中D-乳酸(396.0±9.9 ng/mL)以及DAO(5.4±0.1mg/mL)水平显著高于对照组(D-乳酸:382.0±7.8 ng/mL,DAO:5.0±0.2 mg/mL,P<0.05)。铅中毒能使大鼠菌群多样性下降(P<0.05),同时罗姆布茨菌属、梭菌属、苏黎世杆菌属、棒状杆菌属和寡源杆菌属相对丰度降低,而大肠埃希菌属-志贺菌属和拟杆菌属成为主要特征菌群。结论铅中毒能够改变破坏肠屏障功能和影响肠道菌群。Objective To investigate the effects of lead poisoning on intestinal flora and intestinal barrier function in rats,and to provide new methods for preventing lead poisoning.Methods Three-week-old healthy Wistar male rats were randomly divided into the control and model groups(6 rats for each group).Lead acetate solution(30 mg/kg body weight)was injected intraperitoneally once every other day for 14 days.After that,the serum levels of D-lactate and diamine oxidase(DAO)were detected.The morphology of ileum was observed by HE staining.The content of zonula occludens-1(ZO-1)was detected by immunohistochemical staining.The 16S rRNA sequencing was used to detect the relative abundance of microbes in the ileum.Results The results showed that lead poisoning changed the morphology of intestinal epithelial cells,decreased the level of ZO-1,and destroyed the mucosal barrier.Serum D-lactic acid(396.0±9.9 ng/mL)and DAO(5.4±0.1 mg/mL)levels in lead poisoning group were significantly higher than those in control group(D-lactic acid:382.0±7.8 ng/mL,DAO:5.0±0.2 mg/mL,P<0.05).Lead poisoning reduced the diversity of microbe in rats,and the relative abundance of Romboutsia,Clostridium,Turicibacter,Corynebacterium and Oligella decreased,while Escherichia-Shigella and Bacteroides became the primary characteristic microbe.Conclusion Lead poisoning could damage the intestinal barrier function and alter the intestinal flora.

关 键 词：铅中毒 大鼠 肠道菌群 

分 类 号：R134[医药卫生—劳动卫生]