以人工接菌法研究杀菌剂对水稻叶瘟的预防和治疗效果  

Evaluation of the prevention and control effects of fungicides to rice leaf blast by artificial inoculation method in greenhouse

作　　者：董丽英[1] 陈建晨 刘沛 刘树芳[1] 张先闻 杨勤忠[1] Dong Liying;Chen Jianchen;Liu Pei;Liu Shufang;Zhang Xianwen;Yang Qinzhong(Institute of Agricultural Environment Resource Research,Yunnan Academy of Agricultural Sciences/Key Laboratory of Green Prevention and Control of Agricultural Transboundary Pest of Yunnan Province,Kunming Yunnan 650205,China;School of Agriculture and Life Science,Kunming University,Kunming Yunnan 650214,China)

机构地区：[1]云南省农业科学院农业环境资源研究所/云南省农业跨境有害生物绿色防控重点实验室,云南昆明650205 [2]昆明学院农学与生命科学学院,云南昆明650214

出　　处：《中国植保导刊》2024年第6期5-8,共4页China Plant Protection

基　　金：云南省重大科技专项计划(202102AE090003);国家自然科学基金项目(31860524)。

摘　　要：在温室条件下对苗期水稻进行人工接菌处理,评价3种常用杀菌剂对稻叶瘟的预防和治疗效果。先以供试稻瘟菌HN09-1C-7接种含有不同稻瘟病抗性基因的水稻单基因鉴别系,该菌株对12份水稻单基因鉴别系及供试水稻品种RD23表现出稳定的致病性,可保证后续接菌试验的可靠性,在此基础上分别开展先施药后接菌、先接菌后施药两组试验。结果显示,甲硫·三环唑、稻瘟灵和肟菌·戊唑醇在先施药后接菌试验中对叶瘟的预防效果分别为93.97%、90.06%和99.68%,在先接菌后施药试验中对叶瘟的治疗效果分别为41.80%、67.96%和74.49%。上述药剂用于水稻叶瘟的预防均具较好效果,但用于已发病后的治疗则效果相对较差。本研究提供了一套较为稳定、可靠的人工接菌引发水稻叶瘟方案,并为生产中针对叶瘟的合理用药提供了参考。The prevention and control effects of three commonly used fungicides on rice leaf blast at the seedling-stage were evaluated by artificial inoculation treatments under greenhouse conditions.Magnaporthe oryzae strain HN09-1C-7 was inoculated onto 12 rice monogenic differential lines and the rice cultivar RD23 to evaluate its pathogenicity.M.oryzae strain HN09-1C-7 showed consistent pathogenicity,indicating its suitability for subsequent inoculation experiments.Based on this observation,two experimental groups were designed,one in which fungicides were applied fisrst,followed by inoculation,and another in which inoculation was conducted first,followed by the application of fungicides.The results demonstrated that when the dosages of the effective components of methylthio·tricyclazole,isoprothiolane,and trifloxy-strobin·tebuconazole were 378 mL/hm^(2),600 mL/hm^(2),and 202.5 g/hm^(2),respectively,the control effects on rice leaf blast were93.97%,90.06%,and 99.68% in the fungicides application followed by inoculation group,and 41.80%,67.96%,and 74.49% in the inoculation followed by fungicides application group,respectively.It can be concluded that all three tested fungicides exhibited relatively high preventive effects against rice leaf blast.However,the effect was relatively lower after the disease had occurred.This study provides an important reference for the rational use of leaf blast in rice production.

关 键 词：水稻 叶瘟 人工接菌 杀菌剂 防效 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]