不同生育期和茬次枸杞同位素与元素特征及对溯源的影响  

Study on the Isotopic and Elemental Variances of Wolfberry with Different Maturities and Plucking Times

作　　者：开建荣 张瑶 吕毅 王晓菁 陈畅 王彩艳 苟春林 刘志 KAI Jianrong;ZHANG Yao;LYU Yi;WANG Xiaojing;CHEN Chang;WANG Caiyan;GOU Chunlin;LIU Zhi(Ningxia Research Institute of Quality Standards and Testing Technology of Agricultural Products,Yinchuan,Ningxia 750000;Key Laboratory of Quality and Safety of Wolfberry and Wine for State Administration for Market Regulation/Ningxia Food Testing Institute,Yinchuan,Ningxia 750002;College of Agriculture and Biotechnology,Hunan University of Humanities,Science and Technology,Loudi,Hunan 417000)

机构地区：[1]宁夏农产品质量标准与检测技术研究所,宁夏银川750000 [2]国家市场监督管理总局枸杞与葡萄酒质量安全重点实验室/宁夏食品检测研究院,宁夏银川750002 [3]湖南人文科技学院农业与生物技术学院,湖南娄底417000

出　　处：《核农学报》2024年第9期1724-1733,共10页Journal of Nuclear Agricultural Sciences

基　　金：宁夏回族自治区十四五规划项目(NGSB-2021-5);宁夏农林科学院科技创新引导资金项目(NKYJ-20-14);宁夏回族自治区自然科学基金项目(2021AAC03283)。

摘　　要：为探讨枸杞不同生育期与采摘茬次间稳定同位素分馏和多元素富集差异,评估其变化对产地溯源稳定性的影响尺度,本研究采集宁夏3个产地(固原市、银川市、中宁县)5个生育期(幼果期、青果期、黄变前期、黄变后期及红熟期)和5个采摘茬次的枸杞果样本,测定4个稳定同位素比率和57种元素含量;采用单因素方差分析比较不同生育期和采摘茬次的枸杞稳定同位素比率和元素含量特征差异;建立多元统计偏最小二乘判别分析(PLS-DA)模型,揭示样本类别间的差异尺度。结果表明,不同生育期和茬次的部分稳定同位素比率和多元素含量差异显著。进一步通过PLS-DA产地判别模型发现,虽然不同生育期和茬次的稳定同位素与多元素一定程度上影响聚类,但对产地判别影响较小,3个产地样本总体判别准确率为100%。该研究可为枸杞产地溯源提供理论与技术支撑。This study aims to explore the differences of isotopic fractionation and elemental enrichment of wolfberry between growth periods and plucking times,and to estimate the influence of these changes on origin traceability.Wolfberry fruit samples of 5 growth periods(i.e.,fruitlet,green-fruit,early yellowing,late yellowing and ripe stage)and 5 plucking times were collected in 3 producing areas of Ningxia province(Guyuan,Yinchuan,and Zhongning).Four stable isotope ratios and fifty-seven elemental contents were determined.One-way analysis of variance(ANOVA)were used to analyze the differences between growth periods and plucking times.Next,the partial least squares discriminant analysis(PLS-DA)multivariate statistical method was conducted based on isotopic and elemental data of samples to reveal their different scales between sample groups.Significant differences were observed in theδ13C,δ2H,δ18O,and partial multielemental signatures of wolfberry fruit samples between growth periods and plucking times(P<0.05).However,these variations had little impact on origin discrimination,with an overall discrimination accuracy of 100%for the three origins.This study could provide theoretical explorations and technique supports for the origin traceability of wolfberry.

关 键 词：枸杞 生育期 采摘茬次 稳定同位素及多元素分析 分馏机制 

分 类 号：S567.19[农业科学—中草药栽培]