桉叶油素型和柠檬醛型樟树精油抗氧化研究  

Antioxidant Activity of Eucalyptus-Type and Citral-Type Camphor Essential Oils

作　　者：杨伟 张北红[1] 另青艳 钟庆 余佳敏 肖祖飞 金志农[1] 刘苑秋[3] YANG Wei;ZHANG Beihong;LING Qingyan;ZHONG Qing;YU Jiamin;XIAO Zufei;JIN Zhinong;LIU Yuanqiu(The School of Water and Soil Conservation,Nanchang Institute of Technology/Jiangxi Provincial Engineering Research Center of Seed-breeding and Utilization of Camphor Trees,Nanchang,Jiangxi 330099;College of Horticulture and Forestry Sciences,Huazhong Agricultural University,Wuhan,Hubei 430000;Forestry College,Jiangxi Agricultural University,Nanchang,Jiangxi 330099;Ji’an Landscaping Center,Ji’an,Jiangxi 343000)

机构地区：[1]南昌工程学院水土保持学院/江西省樟树繁育与开发利用工程研究中心,江西南昌330099 [2]华中农业大学园艺林学学院,湖北武汉430000 [3]江西农业大学林学院,江西南昌330099 [4]吉安市园林绿化中心,江西吉安343000

出　　处：《核农学报》2024年第9期1751-1760,共10页Journal of Nuclear Agricultural Sciences

基　　金：江西省科学技术厅重大科技研发专项(20203ABC28W016);江西省林业局林业科技创新项目([2020]07);江西省自然科学基金面上项目(20232BAB205055)。

摘　　要：为探究桉叶油素型和柠檬醛型樟树精油及其纯露的抗氧化性,采用水蒸气蒸馏法提取桉叶油素型和柠檬醛型樟树精油,气相色谱质谱联用分析仪(GC-MS)分析其化学成分及相对含量。结果表明,桉叶油素型樟树精油中相对含量0.1%以上的化学成分有15种,其中桉叶油素相对含量最高为57.28%,精油浓度为128 g·L^(-1)时,对1,1-二苯基-2-三硝基苯肼自由基(DPPH·)清除率(82.60%)高于阳性对照(77.85%),对DPPH·和2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐自由基(ABTS·^(+))清除能力最强的半抑制浓度(IC_(50))分别为14.25、31.65 g·L^(-1)。柠檬醛型樟树精油中相对含量0.1%以上的化学成分有30种,柠檬醛(橙花醛和香叶醛)相对含量最高为42.32%,对DPPH·和ABTS·^(+)清除能力最强的IC_(50)分别为44.45、34.46 g·L^(-1)。精油纯露对DPPH·和ABTS·^(+)有一定的清除效果,且桉叶油素型纯露最优抗氧化性(DPPH·:53.24%,ABTS·^(+):10.50%)强于柠檬醛型纯露(DPPH·:10.58%,ABTS·^(+):9.06%)。两种化学型樟树精油及其纯露均具有抗氧化性,且在一定浓度范围内表现为高浓度精油的抗氧化性显著强于低浓度,桉叶油素型樟树精油抗氧化性强于柠檬醛型。本研究结果可为樟树精油的开发利用提供理论依据。In order to explore the antioxidant properties of eucalyptus and citral camphor essential oils and hydrosols,the eucalyptus and citral camphor essential oils were extracted by steam distillation.Chemical composition and relative content of extracted oils were analyzed by gas chromatography-mass spectrometry analyzer(GC-MS).A total number of 15 major compounds with a relative content of more than 0.1%was found in eucalyptus-type camphor essential oil,among which Eucalyptol had the highest content of 57.28%.At the essential oil concentration of 128 g·L^(-1),the scavenging rate of essential oil to 1,1-diphenyl-2-picrylhydrazyl radical(DPPH·)was 82.60%,which was higher than that of the positive control(77.85%).The half maximal inhibitory concentration(IC50)with the strongest scavenging capacity for DPPH·and 2,2-Azinobis-(3-ethylbenzthiazoline-6-sulphonate)radical(ABTS·^(+))were 14.25 and 31.65 g·L^(-1)respectively.There were 30 major compounds with a relative content more than 0.1%in citral-type camphor essential oil,among which citral(neral and geranial)had the highest content of 42.32%.The IC_(50) with the strongest scavenging capacity for DPPH·and ABTS·^(+)were 44.45 and 34.46 g·L^(-1)respectively.The hydrosols of essential oil had a certain scavenging effect on DPPH and ABTS,and the optimum antioxidant properties of eucalyptus-type hydrosols(DPPH·:53.24%,ABTS·^(+):10.50%)was higher than citral-type hydrosols(DPPH·:10.58%,ABTS·^(+):9.06%).Both chemotypes of camphor essential oils and hrdrosols possessed antioxidant properties,and in a certain concentration range,the antioxidant properties of the essential oils were significantly stronger at higher concentration than the lower concentration.Essential oil of eucalyptustype camphor had stronger antioxidant activity than citral-type.The research provide a theoretical basis for the development and utilization of camphor essential oil.

关 键 词：樟树 精油 桉叶油素 柠檬醛 抗氧化 

分 类 号：TQ645.1[化学工程—精细化工]