微生物种衣剂的制备及其对大麦生长的影响  

Preparation of Microbial Seed Coating Agents and Their Effect on Barley Growth

作　　者：宋忠振 姜子豪 侯晓晴 富洋 俞志敏[1] SONG Zhongzhen;JIANG Zihao;HOU Xiaoqing;FU Yang;YU Zhimin(School of Biological Engineering,Dalian Polytechnic University,Dalian,Liaoning 116034)

机构地区：[1]大连工业大学生物工程学院,辽宁大连116034

出　　处：《核农学报》2024年第9期1798-1804,共7页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金资助(31671796),大连市重点研发计划项目(2022YF16SN064)。

摘　　要：为促进大麦种子萌发及幼苗生长,明确微生物种衣剂在大麦生产中的作用,以黄原胶-明胶(XG-GEL)为成膜剂,解淀粉芽孢杆菌B1(Bacillus amyloliquefaciens B1)为活性成分设计制备微生物种衣剂XG-GEL-B1;通过研究微生物种衣剂对大麦发芽率及幼苗生长和生理指标的影响,以优化微生物种衣剂的使用比例。结果表明,当药种比为1∶75时,成膜剂包衣大麦种子后的发芽率较清水处理组提高12.67%;同时,风干24 h时的细菌活性较B1包衣提升13.92%。进一步研究发现,当成膜剂中的细菌浓度为104 CFU·mL^(-1)时,与清水处理组相比,制备得到的XG-GEL-B1可使发芽24 h时的大麦种子发芽率显著提升14.00个百分点。盆栽试验结果表明,XG-GEL-B1包衣处理使大麦幼苗的株高、根长和鲜重分别较清水处理组提高20.39%、13.51%和14.63%;可溶性总糖、可溶性蛋白、叶绿素a、叶绿素b和叶绿素a+b含量分别较清水处理组提高16.18%、1.97%、13.07%、44.47%和26.47%。此外,本研究制备的XG-GEL-B1种衣剂符合悬浮种衣剂和微生物菌剂的pH值、粘度、成膜时间、包衣均匀度和包衣脱落率指标要求,可用作大麦种子包衣。上述结果表明,微生物种衣剂XG-GEL-B1可提高种子发芽率和作物生理特性。本研究结果为大麦的科学、高效种植提供了理论基础。To promote the germination of barley seeds and enhance seedling growth,while elucidating the role of microbial seed coating agents in barley production,this study designed a novel microbial seed coating agent,which employed xanthan gum-gelatin(XG-GEL)as the film-forming agent and incorporated Bacillus amyloliquefaciens B1 as the bioactive component(XG-GEL-B1).The aim of the study to investigate the effects of microbial seed coating agents on barley germination rate,seedling growth,and physiological indicators is to clarify the proportion of microbial seed coating agents used.The results demonstrated that when the ratio of the coating agent to seeds was 1∶75,the germination rate of barley seeds coated with the film-forming agent was increased by 12.67%.Simultaneously,the activity of B1 bacteria after air-drying for 24 hours increased by 13.92%compared to the B1 coating treatment.Further investigation indicated that with a bacterial concentration of 104 CFU·mL^(-1)in the film-forming agent,the formulated XG-GEL-B1 enhanced the germination rate of barley seeds by 14.00 percentage points after 24 hours,in contrast to the control group treated with water.Pot experiments revealed that XG-GEL-B1 coating agent increased the plant height,root length,and fresh weight of barley seedlings by 20.39%,13.51%,and 14.63%,respectively,compared to the water-treated group.The contents of total soluble sugars,soluble proteins,chlorophyll a,chlorophyll b,and total chlorophyll(a+b)were increased by 16.18%,1.97%,13.07%,44.47%,and 26.47%,respectively,compared to the watertreated group.The XG-GEL-B1 coating agent prepared in this study meeted the standards for pH,viscosity,film formation time,coating uniformity,and coating shedding rates for suspended seed coatings and microbial agents,making it suitable for barley seed coating treatment.The results indicated that XG-GEL-B1 coating agent could enhance seed germination rates and crop physiological characteristics.The findings of this study provide a theoretical foundation for the scient

关 键 词：解淀粉芽孢杆菌 种衣剂 大麦 生理特性 药种比 

分 类 号：S512.3[农业科学—作物学]