短期强噪声对重度耳聋大鼠非听觉系统影响的实验研究  

Effects of short-term intense noise on non-auditory systems of rats with severe deafness

作　　者：彭智勇 王俐钞 丁猛 朱伟 吕传禄 杨阳 王庆蓉 沈俊 Peng Zhiyong;Wang Lichao;Ding Meng;Zhu Wei;Lyu Chuanlu;Yang Yang;Wang Qingrong;Shen Jun(Office of Aviation Medicine,Naval Medical Center,Naval Medical University,Shanghai 200433,China;Unit for Aviation Physiology and Psychology Training,Naval Medical Center,Naval Medical University,Shanghai 200433,China;Office of Ergonomics and Protection Against High-tech Weapons,Naval Medical Center,Naval Medical University,Shanghai 200433,China;Office of Medical Equipment Research,Naval Medical Center,Naval Medical University,Shanghai 200433,China)

机构地区：[1]海军军医大学特色医学中心航空医学研究室,上海200433 [2]海军军医大学特色医学中心航空生理心理训练队,上海200433 [3]海军军医大学特色医学中心人因工程与高新技术武器防护研究室,上海200433 [4]海军军医大学特色医学中心卫生装备与综合论证研究室,上海200433

出　　处：《中华航海医学与高气压医学杂志》2024年第3期377-381,共5页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

基　　金：海军装备科研项目(HJ2018C030376)。

摘　　要：目的:观察短期强噪声对正常听力大鼠和重度耳聋大鼠非听觉系统的影响。方法:按照处理方法将40只SPF级Wistar大鼠分为正常听力组( n=12)、重度耳聋组( n=14)和空白对照组( n=14)。空白对照组大鼠不作任何处理;重度耳聋组大鼠内耳手术造模后和正常听力组大鼠进行强噪声暴露(120 dB白噪声,4 h/d,连续2 d)。观察各组大鼠听性脑干反应(ABR)阈值、体重、血常规、血生化、血肾上腺激素等指标变化,以及大鼠肝脏、胃、肾脏、肺、心脏的病理学改变。 结果:与强噪声暴露前相比,暴露后正常听力组大鼠的ABR阈值明显提高;与造模前相比,造模后重度耳聋组大鼠的ABR阈值明显提高;与正常听力组相比,强噪声暴露后重度耳聋组大鼠的ABR阈值明显提高,差异有统计学意义( P<0.05)。与强噪声暴露前相比,暴露后正常听力组和重度耳聋组大鼠的体重减轻,差异有统计学意义( P<0.05)。强噪声暴露后,与空白对照组和重度耳聋组相比,正常听力组大鼠的血红蛋白(HB)、平均红细胞血红蛋白(MCH)、血小板(PLT)、平均血小板体积(MPV)、淋巴细胞绝对值(LYM)、淋巴细胞百分率(LYM%)、中值细胞绝对值(MID)下降,中性粒细胞绝对值(NEU)升高,血糖(GLU)、尿酸(UA)、乳酸脱氢酶(LDH)、肌酸激酶(CK)水平均降低,血清去甲肾上腺素(NE)和皮质醇(CORT)水平降低;与空白对照组相比,重度耳聋组大鼠的血清NE、CORT水平升高,差异有统计学意义( P<0.05)。重度耳聋组大鼠的肝脏肝窦扩张、炎症细胞浸润;肺脏轻微炎症细胞浸润;肾脏肾小球增生、部分肾小管上皮细胞脱落;心脏和胃未见明显病理变化。正常听力组大鼠的心、肝、肺、胃、肾未见明显病理变化。 结论:短期强噪声暴露后,正常听力组和重度耳聋组大鼠在外周血象、血生化、肾上腺激素及重要脏器病理方面存在不同的变化,噪声的非听觉系统作用途径有待进一ObjectiveTo observe the effects of short-term intense noise on non-auditory systems of rats with normal hearing and with severe deafness.MethodsAccording to the way of intervention,40 SPF Wistar rats were divided into three groups as follows:normal hearing group(n=12),severe deafness group(n=14),and control group(n=14).Rats in the control group did not receive any treatment;rats undergone inner ear surgical modeling in severe deafness group and normal hearing rats were exposed to high intense noise(120 dB white noise,4 h/d,for two consecutive days).Observe the changes in auditory brainstem response(ABR)threshold value,body weight,Complete blood count,blood biochemistry,blood adrenal hormones,and pathological changes in the liver,stomach,kidney,lung,and heart of rats in each group.ResultsABR threshold value of rats in normal hearing group was significantly increased after intense noise exposure;ABR threshold value of rats in severe deafness group was significantly increased after modeling;compared with the normal hearing group,ABR threshold value of rats in severe deafness group after intense noise exposure was significantly increased;all differences were statistically significant(P<0.05).Weight of rats in both normal hearing group and severe deafness group decreased after intense noise exposure,with statistically significant differences(P<0.05).After the noise exposure,compared with the control group and severe deafness group,the value of HB,MCH,PLT,MPV,LYM,LYM%,and MID in the normal hearing group decreased,while the value of NEU increased;but the level of GLU,UA,LDH,CK,serum NE,and CORT decreased.The level of serum NE and CORT in the severe deafness group increased,compared with the control group,with statistically significant differences(P<0.05).The severe deafness group showed microscopic pathological changes such as liver sinusoidal dilation and inflammatory cell infiltration;mild inflammatory cell infiltration in the lungs;renal glomerular hyperplasia,and desquamation of some renal tubular epithelial cells;n

关 键 词：噪声 重度耳聋 非听觉系统 大鼠 

分 类 号：R764.43[医药卫生—耳鼻咽喉科]