Time-of-flight resolved stimulated Raman scattering microscopy using counter-propagating ultraslow Bessel light bullets generation  

作　　者：Shulang Lin Li Gong Zhiwei Huang 

机构地区：[1]Optical Bioimaging Laboratory,Department of Biomedical Engineering,College of Design and Engineering,National University of Singapore,Singapore 117576,Singapore

出　　处：《Light(Science & Applications)》2024年第7期1427-1440,共14页光（科学与应用)（英文版）

基　　金：the Academic Research Fund(AcRF)from the Ministry of Education(MOE)(Tier 2(A-8000117-01-00);Tier 1(R397-000-334-114,R397-000-371-114,and R397-000-378-114);the Merlion Fund(R397-000-356-133),2024 Tsinghua-NUS Joint Research Initiative Fund,and the National Medical Research Council(NMRC)(A-0009502-01-00 and A-8001143-00-00),Singapore.

摘　　要：We present a novel time-of-flight resolved Bessel light bullet-enabled stimulated Raman scattering(B2-SRS)microscopy for deeper tissue 3D chemical imaging with high resolution without a need for mechanical z-scanning.To accomplish the tasks,we conceive a unique method to enable optical sectioning by generating the counterpropagating pump and Stokes Bessel light bullets in the sample,in which the group velocities of the Bessel light bullets are made ultraslow(e.g.,vg≈0.1c)and tunable by introducing programmable angular dispersions with a spatial light modulator.We theoretically analyze the working principle of the collinear multicolor Bessel light bullet generations and velocity controls with the relative time-of-flight resolved detection for SRS 3D deep tissue imaging.We have also built the B2-SRS imaging system and present the first demonstration of B2-SRS microscopy with Bessel light bullets for 3D chemical imaging in a variety of samples(e.g.,polymer bead phantoms,biological samples such as spring onion tissue and porcine brain)with high resolution.The B2-SRS technique provides a>2-fold improvement in imaging depth in porcine brain tissue compared to conventional SRS microscopy.The method of optical sectioning in tissue using counter-propagating ultraslow Bessel light bullets developed in B2-SRS is generic and easy to perform and can be readily extended to other nonlinear optical imaging modalities to advance 3D microscopic imaging in biological and biomedical systems and beyond.

关 键 词：BESSEL RESOLVED PROPAGATING 

分 类 号：O43[机械工程—光学工程]