蓝桉叶片桉叶素生物合成机理研究  

Study on the mechanism of 1,8-cineole biosynthesis in Eucalyptus globulus leaves

作　　者：付朴艳 李华 许玉兰 曹子林[2] 杜官本[3] 王晓丽 FU Puyan;LI Hua;XU Yulan;CAO Zilin;DU Guanben;WANG Xiaoli(College of Forestry,Southwest Forestry University,Kunming 650224,Yunnan,China;School of Ecology and Environment,Southwest Forestry University,Kunming 650224,Yunnan,China;College of Materials and Chemical Engineering,Southwest Forestry University,Kunming 650224,Yunnan,China)

机构地区：[1]西南林业大学林学院,云南昆明650224 [2]西南林业大学生态与环境学院,云南昆明650224 [3]西南林业大学材料与化学工程学院,云南昆明650224

出　　处：《云南大学学报（自然科学版）》2024年第4期776-784,共9页Journal of Yunnan University(Natural Sciences Edition)

基　　金：国家自然科学基金(32260385);云南省种子种业联合实验室项目(202205AR070001-18);云南省教育厅科学研究基金(2021Y247);西南林业大学科研启动基金(112005).

摘　　要：为探明蓝桉(Eucalyptus globulus Labill.)叶片桉叶素合成过程中关键酶活性与桉叶素含量的关系,以不同发育阶段的蓝桉叶片为研究对象,测定桉叶素相对含量和3-羟基-3-甲基戊二酸单酰辅酶A还原酶(HMGR)、1-脱氧-D-木酮糖-5-磷酸合酶(DXS)、异戊烯基焦磷酸异构酶(IPPI)、香叶基焦磷酸合酶(GPPS)、桉叶素合酶(CinS)活性,并对其进行方差分析、相关性分析、通径分析.结果表明,随着蓝桉叶片成熟度增加,桉叶素相对含量逐渐增加,HMGR、DXS、GPPS、IPPI的酶活性逐渐下降.在一个完整的年生长周期内,桉叶素相对含量在7月份显著高于其余月份,HMGR、DXS、GPPS、CinS活性在5、6月份显著高于其余月份.桉叶素相对含量与DXS、CinS活性显著正相关,与HMGR、IPPI、GPPS酶活性极显著负相关.研究结果表明,在5、6月份增强蓝桉幼嫩叶片的DXS和CinS活性,可以有效提高桉叶素积累,进而提高蓝桉桉叶油的产量和品质.To investigate the relationship between key enzyme activity and 1,8-cineole content in the synthesis process of Eucalyptus globulus leaves,this study focuses on the leaves of Eucalyptus globulus at different developmental stages.It measures the relative content of 1,8-cineole and the activities of HMGR,DXS,IPPI,GPPS,and CinS,and conducts analysis of variance,correlation,and path analysis.The results showed that with the increase of leaf maturity of Eucalyptus globulus,the relative content of 1,8-cineole showed an increasing trend,while the enzyme activities of HMGR,DXS,GPPS,and IPPI showed a decreasing trend.During the complete annual growth cycle,the relative content of 1,8-cineole was significantly higher in July than in other months,and the activities of HMGR,DXS,GPPS,and CinS were significantly higher in May and June than in other months.The relative content of 1,8-cineole is significantly positively correlated with DXS and CinS activities,and significantly negatively correlated with HMGR,IPPI,and GPPS enzyme activities.The research results indicate that enhancing the enzyme activities of DXS and CinS in young leaves of Eucalyptus globulus in May and June can effectively increase the accumulation of 1,8-cineole,thereby improving the yield and quality of eucalyptus leaf oil.

关 键 词：蓝桉 桉叶素 3-羟基-3-甲基戊二酸单酰辅酶A还原酶 1-脱氧-D-木酮糖-5-磷酸合酶 异戊烯基焦磷酸异构酶 香叶基焦磷酸合酶 桉叶素合酶 

分 类 号：S792.39[农业科学—林木遗传育种]