烟蚜茧蜂内生细菌对3种烟草害虫的影响  

Effects of endophytic bacteria from Aphidius gifuensis on three tobacco pests

作　　者：黄建文 周挺[2] 顾钢[2] 赖宇飞 杨晨 赖荣泉 张榜 HUANG Jianwen;ZHOU Ting;GU Gang;LAI Yufei;YANG Chen;LAI Rongquan;ZHANG Bang(Biological Control Research Institute/State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops,College of Plant Protection,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;Fujian Province Corporation of China National Tobacco Corporation,Fuzhou,Fujian 350003,China;College of Plant Protection,Shandong Agricultural University,Tai′an,Shandong 271018,China)

机构地区：[1]福建农林大学植物保护学院,生物防治研究所/闽台作物有害生物生态防控国家重点实验室,福建福州350002 [2]中国烟草总公司福建省公司,福建福州350003 [3]山东农业大学植物保护学院,山东泰安271018

出　　处：《武夷科学》2024年第1期45-53,共9页Wuyi Science Journal

基　　金：中国烟草总公司福建省公司项目(2022350000240069);福建省自然科学基金面上项目(2017J01617,2020J01523)。

摘　　要：为探究烟蚜茧蜂内生细菌[短小芽孢杆菌(Bacillus pumilus)、微小杆菌(Exiguobacterium sibiricum)、蜡样芽胞杆菌(Bacillus cereus)(黄色菌落和白色菌落)]对烟蚜(Myzus persicae)、斜纹夜蛾(Spodoptera litura)和烟粉虱(Bemisia tabaci)的影响,本研究采用虫体液浸法和食叶浸渍法分别研究了4种内生细菌对烟蚜的触杀效果和产蚜量、斜纹夜蛾幼虫的发育速率及其取食量、烟粉虱的触杀效果的影响。结果表明,微小杆菌对烟蚜有显著的触杀效果,处理后24 h,稀释倍数为10时,校正死亡率最高为26.67%;微小杆菌对烟蚜产蚜量有显著抑制作用,处理后24 h,稀释倍数为10时,产蚜量最少为3.33头,比对照少了88.65%。4种内生细菌对斜纹夜蛾幼虫的发育速率无显著影响;短小芽孢杆菌对斜纹夜蛾幼虫取食率有显著促进作用,处理后72 h,稀释倍数为10000的促进作用最强,取食率为73.33%,比对照提高了51.66%;蜡样芽胞杆菌(黄色菌落)对斜纹夜蛾幼虫取食率有显著抑制作用,处理后24 h,稀释倍数为10000时,抑制作用最强,取食率为8.33%,抑制率达73.70%。试验结果可为利用内生细菌控制烟草害虫提供科学参考。Insect body fluid immersion and leaf feeding impregnation methods were used to explore the effects of endophytic bacteria[Bacillus pumilus,Exiguobacterium sibiricum and two strains of Bacillus cereus(white and yellow)]on three tobacco pests(Myzus persicae,Spodoptera litura and Bemisia tabaci).The effects of these bacteria on the contact effect and litter size of M.persicae,the larval development and feeding rates of S.litura,and the contact effect of B.tabaci were assessed.The contact effect of E.sibiricum on M.persicae was significant.After 24 h of treatment,the highest corrected mortality was 26.67%at a 1∶10 dilution.E.sibiricum had a significant effect on the litter size of M.persicae.After 24 h of treatment,the minimum litter size of M.persicae was 3.33 individuals,which was reduced by 88.65%for the control at a dilution of 1∶10.However,these bacteria did not significantly affect the larval development rate of S.litura.The feeding rate of B.pumilus on S.litura was increased by 51.66%(73.73%)for the control at a dilution of 1∶10000 after 72 h.It was significant for B.cereus(yellow colony)on feeding rate of S.litura larve.The feeding rate of it was inhibited by 73.70%(8.33%)at a dilution of 1∶10000 after 72 h.These findings demonstrate the potential of endophytic bacteria as a biological control strategy for managing tobacco pests,thereby contributing to more sustainable agricultural practices.

关 键 词：内生细菌 烟蚜 斜纹夜蛾 烟粉虱 害虫防控 可持续农业 

分 类 号：S433.3[农业科学—农业昆虫与害虫防治] Q939.96[农业科学—植物保护]