7种富油微藻的超临界提取及脂肪酸分布特征  

Supercritical Extraction of Lipid from 7 Microalgae Species and Fatty Acid Distribution Characteristics

作　　者：林锡煌 尹希杰 袁秋兰[2] 张锐明 项小燕[2,3] Lin Xihuang;Yin Xijie;Yuan Qiulan;Zhang Ruiming;Xiang Xiaoyan(Third Institute of Oceanography,Ministry of Natural Resources,Xiamen 361005;College of Chemistry and Material Science,Longyan University,Longyan 364000;Fujian Provincial Colleges and University Engineering Research Center of Solid Waste Resource Utilization,Longyan 364000)

机构地区：[1]自然资源部国家第三海洋研究所分析测试中心,厦门361005 [2]龙岩学院化学与材料工程学院,龙岩364000 [3]固体废弃物资源化利用福建省高校工程研究中心,龙岩364000

出　　处：《中国粮油学报》2024年第6期127-132,共6页Journal of the Chinese Cereals and Oils Association

基　　金：福建省教育中青年科技项目(JAT220368);龙岩学院博士启动项目(LB2023007);自然资源部第三海洋研究所基本科研业务费项目(海三科2009055)。

摘　　要：富油微藻是制备生物燃料和提取高附加值不饱和脂肪酸的理想原料。为筛选具有高产油潜能的微藻,开发经济高效的微藻油脂提取工艺和检测方法,研究对7种富油微藻进行超临界CO_(2)提取,并结合三氟化硼-甲醇衍生化、GC-MS分析了各微藻提取物中的35种脂肪酸组成和含量。结果表明,添加乙醇夹带剂后,各藻类的油脂收率由54.60%~82.16%提高至75.61%~104.46%。微藻中共检出18种脂肪酸,C16~C18系列脂肪酸质量分数均在55.97%以上,其中小球藻、三角褐指藻和青岛大扁藻的单不饱和脂肪酸质量分数分别为36.25%、31.81%和26.85%,是生物柴油理想的替代品。牟氏角毛藻的不饱和脂肪酸UFAs质量分数达到91.15%,可作为绿色医疗保健品的重要来源。Microalgae is an ideal raw material for biofuels and green healthy products.To screen the potential biodiesel microalgae and develop a cost-effective lipid extraction and analysis method,7 species of microalgae lipid were extracted using supercritical CO_(2) with ethanol as an entrainer.The composition of 35 fatty acids in microalgae extract were analyzed by boron trifluoride-methanol derivatization and GC-MS analysis.The results indicated that the oil recovery rates of algae increased from 54.60%-82.16%to 75.61%-104.46%after ethanol entrainer is added.A total of 18 types of fatty acids were detected in microalgae samples,mainly were C16 and C18 fatty acids(above 55.97%).Wherein,the content of MUFAs in Chlorella,Phaeodactylum tricornutum,and Platymonas sp.was 36.25%,31.81%and 26.85%,respectively,being ideal substitutes for biodiesel.The content of UFAs in Chaetoceros mueller reached 91.15%,being an important resource of green medical and health products.

关 键 词：富油微藻 超临界CO_(2) 夹带剂 脂肪酸 分布特征 

分 类 号：TQ642[化学工程—精细化工]