Cs4-SeNPs对BV2小胶质细胞炎症反应作用及机制  

Role and mechanism of action of cordyceps polysaccharide-functionalized selenium nanoparticles in inflammatory response of BV2 microglial cells

作　　者：杨妍卿 王晓雯[1] 赵娜娜 张梅[1] 陈文芳[1] YANG Yanqing;WANG Xiaowen;ZHAO Nana;ZHANG Mei;CHEN Wenfang(Department of Physiology and Pathophysiology,School of Basic Medicine,Qingdao University,Qingdao 266071,China)

机构地区：[1]青岛大学基础医学院生理学与病理生理学系,山东青岛266071 [2]山东医学高等专科学校基础医学教学部生理学教研室

出　　处：《青岛大学学报（医学版）》2024年第3期322-326,共5页Journal of Qingdao University(Medical Sciences)

基　　金：国家自然科学基金面上项目(31871144)。

摘　　要：目的 探讨虫草多糖功能化纳米硒(Cs4-SeNPs)对脂多糖(LPS)诱导BV2小胶质细胞炎症反应的作用及其可能机制。方法 以不同浓度(0.01、0.10、1.00μmol/L)Cs4-SeNPs作用LPS诱导的BV2小胶质细胞,采用四甲基偶氮唑蓝(MTT)法检测BV2小胶质细胞活力,免疫印迹技术检测BV2小胶质细胞硒蛋白谷胱甘肽过氧化物酶4(GPX4)蛋白表达,荧光定量PCR技术检测不同时间(4、8、12 h)BV2小胶质细胞促炎因子环氧化酶-2(COX-2)和诱导型一氧化氮合酶(iNOS)mRNA表达。结果 0.01、0.10、1.00μmol/L的Cs4-SeNPs对BV2细胞活力无明显影响。与对照组相比,LPS组GPX4蛋白表达降低(F=25.47,q=6.43,P<0.01);0.01、0.10和1.00μmol/L的Cs4-SeNPs处理组GPX4蛋白表达较LPS组明显升高(q=5.72~14.07,P<0.01),且1.00μmol/L Cs4-SeNPs作用效果最好(q=6.04~8.35,P<0.01)。LPS组COX-2与iNOS mRNA表达较对照组显著上调(F=25.00、37.34,q=12.18、12.06,P<0.001)。1.00μmol/L Cs4-SeNPs预处理12 h可显著抑制COX-2基因表达(q=6.10,P<0.05);预处理8和12 h可显著抑制iNOS mRNA表达(q=4.71、6.97,P<0.05)。结论 Cs4-SeNPs对LPS诱导的BV2小胶质细胞炎症反应具有抑制作用,其机制可能与硒蛋白GPX4的调控有关。Objective To investigate the effect of cordyceps polysaccharide-functionalized selenium nanoparticles(Cs4-SeNPs)on lipopolysaccharide(LPS)-induced inflammatory response of BV2 microglial cells and its possible mechanism.Methods LPS-induced BV2 microglial cells were treated with different concentrations(0.01,0.10,1.00μmol/L)of Cs4-SeNPs.MTT assay was used to measure the viability of BV2 microglial cells;Western blotting was used to measure the protein expression level of the selenoprotein glutathioneperoxidase 4(GPX4)in BV2 microglial cells,and quantitative real-time PCR was used to measure the mRNA expression levels of cyclooxygenase-2(COX-2)and inducible nitric oxide synthase(iNOS)in BV2 microglial cells at diffe-rent time points(4,8,and 12 h).Results Cs4-SeNPs with a concentration of 0.01,0.10,and 1.00μmol/L had no significant in-fluenceon the viability of BV2 cells.Compared with the control group,the LPS group had a significant reduction in the protein expression level of GPX4(F=25.47,q=6.43,P<0.01),and compared with the LPS group,the 0.01,0.10,and 1.00μmol/L Cs4-SeNPs treatment groups had a significant increase in the protein expression level of GPX4(q=5.72-14.07,P<0.01),with 1.00μmol/L Cs4-SeNPs showingthe best effect(q=6.04-8.35,P<0.01).Compared with the control group,the LPS group had significant increases in the mRNA expression levels of COX-2 and iNOS(F=25.00,37.34;q=12.18,12.06;P<0.001).Pretreatment with 1.00μmol/L Cs4-SeNPs for 12 h could significantly inhibit the mRNA expression of COX-2(q=6.10,P<0.05),and pretreatment for 8 and 12 h significantly inhibited the mRNA expression of iNOS(q=4.71,6.97;P<0.05).Conclusion Cs4-SeNPs has an inhibitory effect on LPS-induced inflammatory response in BV2 microglial cells,possibly by regulating the selenoprotein GPX4.

关 键 词：硒 纳米结构 小神经胶质细胞 脂多糖类 炎症 磷脂氢过氧化物谷胱甘肽过氧化物酶 环氧化酶2 

分 类 号：R916.3[医药卫生—药学] R322.8