Exendin-4及内源性GLP-1对大鼠苍白球神经元自发放电活动的调控  

Role of Exendin-4 and endogenous glucagon-like peptide-1 in regulating the spontaneous discharge activity of globus pallidus neurons in rats

作　　者：李筱雪 沈方帅 陈心怡 薛雁 陈蕾 LI Xiaoxue;SHEN Fangshuai;CHEN Xinyi;XUE Yan;CHEN Lei(Department of Physiology and Pathophysiology,School of Basic Medicine,Qingdao University Medical College,Qingdao 266071,China)

机构地区：[1]青岛大学医学部基础医学院生理学与病理生理学系,山东青岛266071 [2]青岛大学附属医院国际医疗中心,山东青岛266071

出　　处：《青岛大学学报（医学版）》2024年第3期327-332,共6页Journal of Qingdao University(Medical Sciences)

基　　金：国家自然科学基金资助项目(32200939);山东省自然科学基金资助项目(ZR2022QC225,ZR2023MC072);泰山学者青年专家计划资助项目(tsqnz20230634)。

摘　　要：目的 探究胰高血糖素样肽-1(GLP-1)受体激动剂Exendin-4及内源性GLP-1对大鼠苍白球神经元自发放电活动的调控。方法 利用在体细胞外电生理记录法,在分别微压力注射浓度均为10μmol/L的Exendin-4和GLP-1受体阻断剂Exendin-9-39后,观察二者对正常大鼠苍白球神经元自发放电活动的影响。结果 在记录到的16个正常大鼠苍白球神经元中,微压力注射Exendin-4可以显著增加其中11个神经元的自发放电频率(t=-4.729,P=0.001),与生理盐水对照组相比差异有显著性(Z=2.776,P<0.01),且可以显著改变其放电模式(t=-3.136,P<0.05),放电规律性降低。在记录到的9个正常大鼠苍白球神经元中,微压力注射Exendin-9-39可显著降低其中6个神经元的自发放电频率(t=6.374,P=0.001),与生理盐水对照组相比差异有显著性(Z=-2.739,P<0.01),但不改变其放电模式。结论 Exendin-4可增加苍白球神经元自发放电频率并改变其放电模式,内源性GLP-1参与了苍白球神经元自发放电活动的调节。Objective To investigate the role of Exendin-4,a glucagon-like peptide-1(GLP-1)receptor agonist,and endo-genous GLP-1 in regulating the spontaneous discharge activity of globus pallidus neurons inrats.Methods In vivo extracellular electrophysiological recordings were performed to observe the effect of micro-pressure injection of 10μmol/L Exendin-4 or 10μmol/L Exendin-9-39,a GLP-1 receptor antagonist,on the spontaneous discharge activity of globus pallidus neurons in normal rats.Results As for the 16 globus pallidusneurons of normal rats recorded,micro-pressure injection of Exendin-4 significantly increased the spontaneous discharge frequency of 11 neurons(t=-4.729,P=0.001),showing a significant difference compared with the normal saline control group(Z=2.776,P<0.01),and it significantly changed the discharge pattern(t=-3.136,P<0.05),with a reduction in the regularity of discharge.As for the 9 globus pallidusneurons of normal rats recorded,micro-pressure injection of Exendin-9-39 significantly reduced the spontaneous discharge frequency of 6 neurons(t=6.374,P=0.001),showing a significant difference compared with the normal saline control group(Z=-2.739,P<0.01),but it didnot change the discharge pattern.Conclusion Exendin-4 increases the spontaneous discharge frequency of globus pallidus neurons and changes their discharge pattern,and endogenous GLP-1 is involved in the modulation of the spontaneous discharge activity of globus pallidus neurons.

关 键 词：苍白球 胰高血糖素样肽-1受体 艾塞那肽 微电极 电生理学 大鼠 WISTAR 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学] R977.15[医药卫生—基础医学]