FAC对小鼠嗅球小胶质细胞和星形胶质细胞的影响  

Effects of FAC on microglia and astrocytes in the olfactory bulb of mice

作　　者：李晖[1] 王娟[1] 王友翠 谢俊霞[1] LI Hui;WANG Juan;WANG Youcui;XIE Junxia(Department of Physiology and Pathophysiology,School of Basic Medicine,Qingdao University Medical College,Qingdao 266071,China)

机构地区：[1]青岛大学基础医学院生理学与病理生理学系,山东青岛266071

出　　处：《青岛大学学报（医学版）》2024年第3期333-336,共4页Journal of Qingdao University(Medical Sciences)

基　　金：国家自然科学基金面上项目(32170984)。

摘　　要：目的 探究经鼻给予枸橼酸铁铵(FAC)对小鼠嗅觉及嗅球(OB)中小胶质细胞和星形胶质细胞的影响。方法 取健康雄性8周龄C57BL/6小鼠20只,随机分成对照组和FAC组,每组10只。FAC组小鼠按照200 mg/kg体质量双侧鼻孔交替滴入FAC溶液,对照组给予等体积生理盐水,两组均隔天滴1次,共2周。2周后测试小鼠嗅觉功能,采用免疫荧光染色方法观察经鼻给铁对OB中小胶质细胞和星形胶质细胞的影响。结果 与对照组相比,FAC组小鼠出现明显的嗅觉障碍,差异有统计学意义(t=9.57,P<0.05);FAC组小鼠OB的质量明显降低,差异有统计学意义(t=10.50,P<0.05);FAC组小鼠OB中激活的小胶质细胞和星形胶质细胞明显增多,差异有统计学意义(t=5.07、4.83,P<0.05)。结论 经鼻给FAC能够引起嗅觉障碍,造成OB损伤以及小胶质细胞和星形胶质细胞激活。Objective To investigate the effects of intranasal administration of ferric ammonium citrate(FAC)on the olfactory function and microglia and astrocytes in the olfactory bulb(OB)of mice.Methods Twenty healthy 8-week-old male C57BL/6 mice were randomly divided into control group and FAC group,with tenmice in each group.The FAC group was treated with FAC solution through the nostrils alternately at a dose of 200 mg/kg,while the control group was given an equal volume of normal saline,once every other day for two weeks.Then,the olfactory function of the mice was tested,and the effects of intranasal administration of iron on microglia and astrocytes in the OB were examined with immunofluorescence staining.Results Compared with the control group,the FAC group showed significant reductionsin olfactory function(t=9.57,P<0.05)and OB weight(t=10.50,P<0.05)and significant increases in activated microglia(t=5.07,P<0.05)and astrocytes(t=4.83,P<0.05)in the OB.Conclusion Intranasal administration of FAC can cause olfactory dysfunction,impair the OB,and promote the activation of microglia and astrocytes in the OB.

关 键 词：铁 嗅球 嗅觉障碍 小胶质细胞 星形胶质细胞 

分 类 号：R591.1[医药卫生—内科学] R338.3[医药卫生—临床医学]