胶质瘤组织BSN表达及其对病人预后的影响  

Expression of the presynaptic cytomatrix protein Bassoon in glioma tissue and its influence on the prognosis of patients

作　　者：周晓彤 傅琳 姜宏[1] ZHOU Xiaotong;FU Lin;JIANG Hong(State Key Disciplines:Physiology(Incubation),Department of Physiology,Qingdao University,Qingdao 266071,China)

机构地区：[1]青岛大学国家生理学重点(培育)学科,山东青岛266071

出　　处：《青岛大学学报（医学版）》2024年第3期345-349,共5页Journal of Qingdao University(Medical Sciences)

基　　金：国家自然科学基金面上项目(32171131);山东省自然科学基金重大基础研究项目(ZR2019-ZD31)。

摘　　要：目的 探究巴松管突触前细胞基质蛋白(BSN)在胶质瘤组织表达及其对病人预后的影响。方法 通过从癌症基因组图谱(TCGA)、中国脑胶质瘤图谱(CGGA)、胶质瘤基因组数据库(GEO)和临床蛋白质组肿瘤分析数据库(CPTAC)下载胶质瘤的转录组数据、蛋白组数据和临床样本资料,分析胶质瘤组织BSN的表达水平及其与病人预后的关系。使用siRNA转染U251细胞株,构建BSN表达下调的胶质瘤细胞系。采用Western blotting技术检测BSN对胶质瘤细胞增殖相关蛋白PCNA和迁移相关蛋白CD44的表达水平的影响。结果 与正常脑组织相比,胶质瘤组织BSN mRNA的表达水平显著下调(P<0.001),其异常低表达与胶质瘤病人的不良预后显著相关(P<0.001)。随着胶质瘤级别的增加,BSN的mRNA水平逐渐下调。在高级别胶质瘤中,BSN的蛋白表达水平也显著低于正常组(P<0.001)。Western blotting检测结果显示,敲低BSN基因可以显著上调U251细胞中PCNA和CD44蛋白的表达水平,差异有统计学意义(F=14.56、164.90,P<0.01)。结论 BSN在胶质瘤组织中表达下调并与肿瘤的预后相关。敲低BSN可以促进胶质瘤细胞增殖、迁移相关蛋白的表达。BSN可能在胶质瘤发生发展中扮演抑癌基因的角色。Objective To investigate the expression of Bassoon(BSN),a presynaptic cytomatrix protein,in glioma tissue and its influence on the prognosis of patients.Methods TCGA,CGGA,GEO,and CPTAC databases were used to download transcriptomic data,proteomic data,and the data of clinical samples,and the expression level of BSN in glioma tissue and its association with prognosis were analyzed.The U251 cell line was transfected with siRNA to construct a glioma cell line with downregulated BSN expression.Western blotting was used to measure the influence of BSN on the expression levels of the proliferation-related protein PCNA and the migration-related protein CD44 in glioma cells.Results The mRNA expression level of BSN was downregulated in glioma tissue compared with normal brain tissue(P<0.001),and the abnormally low expression of BSN was significantly associated with the poor prognosis of glioma patients(P<0.001).The mRNA expression level of BSN gradually decreased with the increase in the grade of glioma.In addition,the protein expression level of BSN in high-grade glioma was significantly lower than that in the normal group(P<0.001).Western blotting showed that knockdown of BSN significantly upregulated the protein expression levels of PCNA and CD44 in U251 cells(F=14.56,164.90;P<0.01).Conclusion BSN expression is downregulated in glioma and is associated with tumor prognosis.Knockdown of BSN can promote the expression of proliferation and migration-related proteins in glioma cells,and BSN may play the role of a tumor suppressor gene in the development and progression of glioma.

关 键 词：神经胶质瘤 巴松管突触前细胞基质蛋白 受体 突触前 胶质母细胞瘤 基因敲低技术 预后 

分 类 号：R338.2[医药卫生—人体生理学]