锌原卟啉对BV2小胶质细胞凋亡相关蛋白表达影响  

Effect of zinc protoporphyrin on the expression of apoptosis-related proteins in BV2 microglial cells

作　　者：刘荣 荆翠婷 谢俊霞[2] 王俊 LIU Rong;JING Cuiting;XIE Junxia;WANG Jun(Department of Physiology,School of Basic Medicine,Qingdao University Medical College,Qingdao 266071,China)

机构地区：[1]青岛大学医学部基础医学院生理学教研室,山东青岛266071 [2]青岛大学脑科学与疾病研究院

出　　处：《青岛大学学报（医学版）》2024年第3期368-371,共4页Journal of Qingdao University(Medical Sciences)

基　　金：山东省重点研发课题(2019GSF108224)。

摘　　要：目的 探究血红素加氧酶1(HO-1)抑制剂锌原卟啉(ZnPP)对BV2小胶质细胞抗凋亡相关蛋白表达水平的影响。方法 以25μmol/L的ZnPP处理BV2小胶质细胞24 h后,使用CCK-8法检测细胞活力,采用Western blot的方法检测细胞内ZnPP对BV2小胶质细胞抗凋亡蛋白B淋巴细胞瘤-2蛋白(Bcl-2)与促凋亡蛋白Bcl2-Associated X蛋白(Bax)、剪切的天冬氨酸特异性半胱氨酸蛋白酶-3(Cleaved caspase 3)等蛋白表达的影响。结果 与对照组相比,以10μmol/L的ZnPP处理BV2小胶质细胞24 h后细胞活力无明显变化(F=14.720,q=2.819,P>0.05)。而以25μmol/L的ZnPP作用于小胶质细胞24 h后,细胞活力明显下降(q=7.591,P<0.001);细胞内HO-1和Bcl-2蛋白表达明显降低,Cleaved caspase 3蛋白和Bax蛋白表达明显增加,差异均有统计学意义(t=2.975~5.189,P<0.01)。结论 ZnPP处理BV2小胶质细胞后可通过线粒体凋亡途径激活细胞凋亡,此过程可能与抑制HO-1的作用有关。Objective To investigate the effect of zinc protoporphyrin(ZnPP),an inhibitor ofheme oxygenase 1(HO-1),on the expression levels of the anti-apoptosis-related proteins in BV2 microglial cells.Methods BV2 microglial cells were treated with 25μmol/L ZnPP for 24 hours.CCK-8 assay was used to measure cell viability,and Western blot was used to measure the changes in the protein expression levels of the anti-apoptotic protein B-cell lymphoma-2(Bcl-2)and the pro-apoptotic proteins Bcl-2 related X protein(Bax)and Cleaved caspase-3 induced by ZnPP in BV2 microglial cells.Results Compared with the control group,the BV2 microglial cells treated with 10μmol/L ZnPP for 24 hours showed no significant change in cell viability(F=14.720,q=2.819,P>0.05),while the microglial cells treated with 25μmol/L ZnPP for 24 hours showed a significant reduction in cell viability(q=7.591,P<0.01),as well as significant reductions in the protein expression levels of HO-1 and Bcl-2 and significant increases in the protein expression levels of Cleaved caspase-3 and Bax(t=2.975-5.189,P<0.01).Conclusion Treatment of BV2 microglial cells with ZnPP can activate cell apoptosis through the mitochondrial apoptosis pathway,possibly by inhibiting HO-1.

关 键 词：锌 原卟啉类 血红素加氧酶-1 小神经胶质细胞 细胞凋亡 线粒体蛋白质类 

分 类 号：R977.9[医药卫生—药品] R322.8[医药卫生—药学]