miRNA-137及其靶蛋白COX-2在扁平苔藓中的表达  

Expression of miRNA-137 and its target protein cyclooxygenase-2 in lichen planus

作　　者：李媛媛[1] 王君[2] 王莹莹[2] 梁淑雅 陆晓鸥[2] LI Yuanyuan;WANG Jun;WANG Yingying;LIANG Shuya;LU Xiaoou(Department of Dermatology,Qingdao Women and Children’s Hospital,Qingdao University,Qingdao 266034,China)

机构地区：[1]青岛大学附属青岛市妇女儿童医院皮肤科,山东青岛266034 [2]青岛大学附属医院皮肤科,山东青岛266034

出　　处：《青岛大学学报（医学版）》2024年第3期425-428,共4页Journal of Qingdao University(Medical Sciences)

基　　金：山东省医药卫生科技发展计划(2016WS0281)。

摘　　要：目的 探讨环氧化酶-2(COX-2)及其靶向miRNA-137在扁平苔藓(LP)中的表达及潜在作用。方法采用实时荧光定量PCR(RT-PCR)法检测40例LP皮损组织中miRNA-137表达情况,以35例正常皮肤组织作为对照。采用酶联免疫吸附测定(ELISA)法检测COX-2在LP组织及正常皮肤组织中的表达水平。结果 与正常皮肤组织相比,LP组织样本COX-2表达明显升高(t=2.163,P=0.034),miRNA-137表达显著下调(t=2.122,P=0.037)。Spearman秩相关分析结果显示,LP组织中miRNA-137与COX-2的表达呈负相关(r=-0.244,P=0.035)。结论 miRNA-137表达下调导致其靶蛋白COX-2的表达上调,这可能在LP发病机制中发挥重要作用,miRNA-137可能会成为LP分子治疗的潜在工具。Objective To investigate the expression and potential role of miRNA-137 and its target cyclooxygenase-2(COX-2)in lichen planus(LP).Methods Quantitative real-time PCR was used to measure the expression of miRNA-137 in skin lesion tissue of 40 patients with LP,and 35 cases of normal skin tissue were selected as the control group.ELISA was used to measure the expression level of COX-2 in LP tissue and normal skin tissue.Results Compared with normal skin tissue,there was a significant increase in the expression level of COX-2 in LP tissue(t=2.163,P=0.034)and a significant reduction in the expression level of miRNA-137(t=2.122,P=0.037).The Spearman’s rank correlation analysis showed that the expression of miRNA-137 was negatively correlated with that of COX-2 in LP tissue(r=-0.244,P=0.035).Conclusion The downregulated expression of miRNA-137 leads to the upregulated expression of its target protein COX-2,which may play an important role in the pathogenesis of LP,and miRNA-137 may become a potential tool for molecular therapy in LP.

关 键 词：扁平苔癣 微RNAS 环氧化酶2 

分 类 号：R758.65[医药卫生—皮肤病学与性病学]