IFN-γ、MMP-9水平在肺结核中的表达及与其病情活动性的相关性  

Expression of IFN⁃γand MMP⁃9 in pulmonary tuberculosis and its correlation with disease activity

作　　者：王霞[1] 黄健[2] 牛文一[1] 赵桂增 姜玉华 韩伟[1] WANG Xia;HUANG Jian;NIU Wenyi;ZHAO Guizeng;JIANG Yuhua;HAN Wei(Department of Tuberculosis,the First Affiliated Hospital of Xinxiang Medical College,Xinxiang,Henan,China,453100;Respiratory Endoscopy Room,the First Affiliated Hospital of Xinxiang Medical College,Xinxiang,Henan,China,453100)

机构地区：[1]新乡医学院第一附属医院结核内科,河南新乡453100 [2]新乡医学院第一附属医院呼吸内镜室,河南新乡453100

出　　处：《分子诊断与治疗杂志》2024年第7期1372-1376,共5页Journal of Molecular Diagnostics and Therapy

基　　金：河南医学科技攻关计划项目(LHGJ20220594)。

摘　　要：目的 分析血清干扰素-γ(IFN-γ)、基质金属蛋白酶-9(MMP-9)水平在肺结核患者中的表达及与其病情活动性的相关性。方法 选取2019年1至2022年12月新乡医学院第一附属医院收治的264例疑似肺结核患者作为研究对象,经确诊后分为结核组(n=204例)及非结核组(n=60例),根据病情活动情况将结核组分为活动性肺结核组(n=86例)及非活动性肺结核组(n=118例),另选取同期于本院进行健康体检者80名为健康组。比较结核组、非结核组、健康组与结核组中活动性肺结核组、非活动性肺结核组及活动性肺结核中轻症组、重症组间血清IFN-γ、MMP-9水平;分析IFN-γ、MMP-9单一及联合检测肺结核的诊断效能;采用多元Logistic回归分析影响活动性肺结核患者病情程度的相关性因素。结果 三组血清IFN-γ、MMP-9水平比较:结核组>非结核组>健康组,差异具有统计学意义(P<0.05);活动性肺结核患者血清IFN-γ、MMP-9水平均显著高于非活动性肺结核,差异有统计学意义(P<0.05);86例活动性肺结核患者中轻症54例,重症32例。重症患者病程比轻症患者长,吸烟比例及血清IFN-γ、MMP-9水平均显著高于轻症患者,差异有统计学意义(P<0.05);IFN-γ、MMP-9联合诊断肺结核的灵敏度、特异度、准确度分别为93.55%、61.54%、84.09%,均高于三者单独检测(P<0.05);经Logistic回归分析,吸烟、血清IFN-γ及MMP-9均为影响活动性肺结核患者病情程度的相关因素(P<0.05)。结论 肺结核患者血清IFN-γ及MMP-9水平显著升高,二者联合对肺结核具有较好的诊断效能,且为影响活动性肺结核病情严重程度的重要影响因素,对肺结核病情评估具有重要价值。Objective To analyze serum interferon-γ(Interferon gamma,IFN-γ),the expression of matrix metalloproteinase-9(MMP-9)levels in pulmonary tuberculosis and its association with disease activ-ity.Methods 264 suspected pulmonary tuberculosis patients admitted to the First Affiliated Hospital of Xinx-iang Medical College from January 2019 to December 2022 were selected as the subjects of the study.After diag-nosis,they were divided into a tuberculosis group(n=204 cases)and a non-tuberculosis group(n=60 cases).The tuberculosis group was further categorized based on disease activity into an active pulmonary tuberculosis group(n=86 cases)and a non-active pulmonary tuberculosis group(n=118 cases).In addition,80 healthy indi-viduals who underwent physical examinations in our hospital during the same period were selected as the control group.The serum levels of IFN-γand MMP-9 were compared between the active pulmonary tuberculosis group,the non-active pulmonary tuberculosis group,and the mild and severe active tuberculosis subgroups within the active tuberculosis group.The diagnostic efficacy of IFN-γand MMP-9,both individually and in combination,for detecting pulmonary tuberculosis was analyzed.The serum levels of IFN-γand MMP-9 in pa-tients with mild and severe cases of active pulmonary tuberculosis were compared,and the correlation factors affecting the severity of active pulmonary tuberculosis patients were analyzed by multivariate logistic regres-sion.Results The comparison of serum IFN-γand MMP-9 levels among three groups showed that the tuber-culosis group>non tuberculosis group>healthy group,and the differences among the three groups were statis-tically significant(P<0.05).The sensitivity,specificity,and accuracy of combined diagnosis using MMP-9 were 93.55%,61.54%,and 84.09%,respectively,which were higher than those detected separately by the three methods(P<0.05).Among 204 pulmonary tuberculosis patients,86 were active pulmonary tuberculosis patients,and 118 were non-active pulmonary tuberculosis patients.The

关 键 词：干扰素-Γ 基质金属蛋白酶-9 肺结核 

分 类 号：R521[医药卫生—内科学]