高表达抗β2糖蛋白I(β2GPI)自身抗体可加重结缔组织病炎症程度及免疫功能紊乱  

High expression of anti-β2 glycoprotein I(β2GPI)autoantibody exacerbates inflammation and immune dysfunction in connective tissue disease

作　　者：万磊[1,2,3] 刘健 黄传兵[1,2] WAN Lei;LIU Jian;HUANG Chuanbing(First Affiliated Hospital of Anhui University of Chinese Medicine,Hefei 230031;Center for Xin’an Medicine and Modernization of Traditional Chinese Medicine of IHM,Hefei 230012;Key Laboratory of Xin’an Medical Education Ministry,Hefei 230012,China)

机构地区：[1]安徽中医药大学第一附属医院,安徽合肥230031 [2]新安医学与中医药现代化研究所,安徽合肥230012 [3]新安医学教育部重点实验室,安徽合肥230012

出　　处：《细胞与分子免疫学杂志》2024年第6期532-537,共6页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然基金面上项目(82274501);新安医学与中医药现代化研究所揭榜挂帅项目(2023CXMMTCM020,2023CXMMTCM004);国家中医药管理局第七批全国老中医药专家学术经验继承项目(国中医药人教函[2022]76号);安徽省临床医学研究转化专项项目(202304295107020114);安徽省自然基金项目(2308085MH291);安徽省高校优秀青年人才支持计划项目(皖教秘人[2022]11号);新安医学教育部重点实验室项目(2020xayx04);安徽省高校自然科学研究项目(2023AH050814);安徽省高等学校质量工程项目省级教育教学改革研究项目(2022jyxm883)。

摘　　要：目的 观察抗β2糖蛋白I(β2GPI)自身抗体在结缔组织病表达及其与炎症及免疫功能的关系。方法 观察对象为广义结缔组织病患者,包括结缔组织病(CTD)、类风湿性关节炎(RA)、干燥综合征(SS)和系统性红斑狼疮(SLE)。采用化学发光法进行定量检测抗β2GPI。采用魏氏法检测血沉(ESR),采用全自动生化分析仪检测C反应蛋白(CRP)、类风湿因子(RF)和抗环瓜氨酸多肽(CCP)抗体。结果 RA患者抗β2GPI抗体及其亚型较CTD、 SS、 SLE患者均明显升高。结缔组织病患者中CRP与抗β2GPI抗体、抗β2GPI抗体IgM呈正比。ESR与抗β2GPI抗体呈正比。与CRP正常组比较,CRP异常组抗β2GPI抗体、抗β2GPI抗体IgM升高。与ESR正常组比较,ESR异常组抗β2GPI抗体、抗β2GPI抗体IgG升高。RA患者中抗β2GPI抗体与ESR、抗CCP抗体呈正相关。抗β2GPI抗体IgG与RF呈正相关。结论 抗β2GPI抗体水平可作为结缔组织病炎症程度和评估免疫紊乱的一个预测因子。Objective To observe the expression of anti-β2 glycoprotein I(β2GPI)autoantibody in connective tissue diseases and its relationship with the degree of inflammation and immune function.Methods Patients with broad connective tissue diseases including connective tissue disease(CTD),rheumatoid arthritis(RA),Sjögren’s syndrome(SS),and systemic lupus erythematosus(SLE)were observed.β2GPI was quantified by chemiluminescence,ESR was measured by Weil’s method,and C-reactive protein(CRP),rheumatoid factor(RF),anti-cyclic citrullinated polypeptide(CCP)antibody were measured by automatic biochemical analyzer.Resultsβ2GPI and their subtypes were significantly higher in RA patients compared with CTD,SS,and SLE patients.CRP was positively associated with anti-β2GPI antibody and anti-β2GPI antibody IgM in patients with connective tissue disease.ESR was positively associated with anti-β2GPI antibody.Anti-β2GPI antibody and anti-β2GPI antibody IgM were elevated in the abnormal CRP group compared with the normal CRP group.Compared with the ESR normal group,anti-β2GPI antibody and anti-β2GPI antibody IgG were elevated in the ESR abnormal group.Anti-β2GPI antibody was positively correlated with ESR and anti-CCP antibody in RA patients.Anti-β2GPI antibody IgG was positively correlated with RF.Conclusionβ2GPI can be used as a predictor of the degree of inflammation and assessment of immune disorders in CTD.

关 键 词：β2糖蛋白I(β2GPI) 结缔组织病 炎症程度 免疫功能 类风湿性关节炎 

分 类 号：R593.22[医药卫生—内科学] R593.2[医药卫生—临床医学] R364.5R392.1