CA12-5联合月经血结核分枝杆菌检测对女性生殖系统结核诊断价值研究  

Diagnostic value of CA12-5 combined with mycobacterium tuberculosis detection in menstrual blood in female reproductive system tuberculosis

作　　者：方喆 王起 袁甜 陈红梅 FANG Zhe;WANG Qi;YUAN Tian;CHEN Hongmei(Tuberculosis Prevention and Control Institute(Hunan Chest Hospital),Changsha,Hunan 410006)

机构地区：[1]湖南省结核病防治所/湖南省胸科医院内一科,湖南长沙410006

出　　处：《智慧健康》2024年第12期146-150,共5页Smart Healthcare

基　　金：湖南省科技创新计划项目《月经血结核杆菌检测在早期诊断生殖器结核的意义》(项目编号:2020SK50702)。

摘　　要：目的探讨月经血结核分枝杆菌荧光定量PCR检测(fluorescent quantitative PCR,FQ-PCR)联合血清糖类抗原12-5(CA12-5)对女性生殖器结核(female genital tuberculosis,FGT)的诊断价值。方法回顾性分析2018年1月—2021年12月本院收治的160例输卵管性及不明原因不孕、复发性流产者,根据内镜下所见表现及病理检测结果,分析月经血结核分枝杆菌FQ-PCR及外周血清CA12-5含量(<35U/L)的检测效能,同时比较与血清结核分枝杆菌FQ-PCR阳性率及检测时长方面的优势。结果FQ-PCR及CA12-5联合检测对生殖器结核漏诊率为12.82%,低于月经血结核分枝杆菌FQ-PCR检测单独检测的64.10%与CA12-5的35.90%以及血清结核分枝杆菌FQ-PCR的7.18%,同时联合检测时间低于常规检查(培养及胸腔镜下检查)时间(均P<0.05)。结论无创月经血结核分枝杆菌FQ-PCR检测联合外周血清CA12-5在女性生殖器结核诊断方面较单一检测手段诊断效能高,漏诊率低,缩短检测时长,具有一定的临床实用价值。Objective To investigate fluorescent quantitative PCR(FQ-PCR)detection of mycobacterium tuberculosis in menstrual blood combined with serum carbohydrate antigen 12-5(CA-12-5)for female genital tuberculosis(female genital tuberculosis)Diagnostic value of tuberculosis(FGT).Methods Clinical examination data of 160 cases of tubal infertility with unknown causes admitted to our hospital from January 2018 to December 2021 were retrospectively analyzed.FQ-PCR and serum CA12-5 content(<35U/L)in menstrual blood were analyzed based on endoscopic findings and pathological findings.At the same time,the positive rate and detection time of FQ-PCR were compared with that of serum mycobacterium tuberculosis.The Results The missed diagnosis rate of genital tuberculosis by FQ-PCR combined with CA12-5 was 12.82%,which was lower than 64.10%by FQ-PCR alone,35.90%by CA12-5 and 7.18%by FQ-PCR in serum.Meanwhile,the combined detection time was lower than that of routine examination(culture and thoracoscopic examination)(both P<0.05).Conclusion Compared with a single detection method,FQ-PCR combined with Wai-Qing CA12-5 has higher diagnostic efficiency in the diagnosis of female genital tuberculosis,lower missed diagnosis rate and less detection time,which has certain clinical practical value.

关 键 词：女性生殖系统结核 月经血结核分枝杆菌 糖类抗原12-5 诊断价值 输卵管性不孕 荧光定量 

分 类 号：R527.4[医药卫生—内科学] R446.5[医药卫生—临床医学]