通脉消斑汤上调PPARα抑制NF-кB减轻缺氧复氧受损心肌细胞炎症反应  

Approach to mechanism of Tongmai Xiaoban decoction protecting hypoxia and reoxygenation-damaged myocardial cells from inflammatory reaction by up-regulating PPARαand inhibiting NF-кB

作　　者：赵海梅 程红[1] 焦萁荟 ZHAO Hai-mei;CHENG Hong;JIAO Qi-hui(Shenzhen Hospital of Traditional Chinese Medicine,Guangdong Province,Shenzhen518033,China)

机构地区：[1]广东省深圳市中医院,深圳518033

出　　处：《山西中医》2024年第8期56-60,共5页Shanxi Journal of Traditional Chinese Medicine

基　　金：广东省深圳市科技计划项目(编号:JCYJ20220531091817039)。

摘　　要：目的:探讨通脉消斑汤上调过氧化物酶体增生因子活化受体α(PPARα)抑制NF-кB保护缺氧复氧受损心肌细胞炎症反应。方法:建立大鼠H9C2细胞缺氧复氧模型,将细胞分为3组:对照组(Ⅰ组)、缺氧复氧组(Ⅱ组)、缺氧复氧加通脉消斑汤组(Ⅲ组)。CCK8检测不同浓度通脉消斑汤对大鼠H9C2活力影响;缺氧复氧以及加用通脉消斑汤对细胞活力的影响。免疫荧光观察PPAR缺氧复氧前后及加用通脉消斑汤后变化,NF-кB P65核转移以及磷酸化NF-κB P65(p-NF-κB P65)胞核内表达变化。Western Blot检测PPAR、p-NF-κB、NF-κB蛋白表达的变化。结果:与空白对照组比较,缺氧复氧组H9C2细胞活力降低,加用通脉消斑汤后,细胞活力升高(P﹤0.05)。免疫荧光显示缺氧复氧组细胞PPAR核内表达较空白对照组降低,加用通脉消斑汤组细胞,PPAR较前升高(P﹤0.05);缺氧复氧组细胞出现NF-κB P65核转移,通脉消斑汤组核转移减少;与空白对照组比较,磷酸化NF-κB在缺氧复氧组表达升高,加用通脉消斑汤组降低。Western Blot结果显示PPAR在缺氧复氧组表达较空白对照组降低,在通脉消斑汤升高;磷酸化NF-κB、细胞间粘附分子1(ICAM1)在缺氧复氧组表达升高,同样在通脉消斑汤组降低。结论:通脉消斑汤上调PPARα,抑制NF-κB,保护缺氧复氧受损心肌细胞。Objective:To approach the mechanism of Tongmai Xiaoban decoction protecting hypoxia and reoxygenation-damaged myocardial cells from inflammatory reaction by up-regulating peroxisome proliferator-activated receptorα(PPARα)and inhibiting NF-кB.Methods:Hypoxia-reoxygenation model of rat H9C2 cells was established.H9C2 cells were divided into 3 groups:blank control group(Group I),hypoxia-reoxygenation group(Group II),hypoxia-reoxygenation adding Tongmai Xiaoban decoction group(Group III).The effect of Tongmai Xiaoban decoction with different concentrations on viability of rat H9C2 cells was tested by CCK8;in addtion,the effects of hanoxia-reoxygenation and adding Tongmai Xiaoban decoction on cell viability were tested.The changes of PPARαwere observed by immunofluorescence method before and after hanoxia-reoxygenation and after adding Tongmai Xiaoban decoction;in addtion,NF-κB P65 nucleus transferring and the change of intracellular expression of phosphorylated NF-κB P65(p-NF-κB P65)were observed.The changes of expressions of PPARα,p-NF-κB and NF-κB proteins were detected by Western Blot.Results:Compared with blank control group,H9C2 cell viability was decreased in hypoxia-reoxygenation group,while the cell viability was increased after adding Tongmai Xiaoban decoction(P﹤0.05).The immunofluorescence results showed that the intracellular expression of cell PPARαin hypoxia-reoxygenation group was decreased compared with in blank control group,while the expression of cell PPARαin adding Tongmai Xiaoban decoction group was increased compared with former(P﹤0.05);The cells in hypoxia-reoxygenation group showed NF-κB P65 nucleus transferring,while the nucleus transferring in addingTongmai Xiaoban decoction group was decreased.Compared with blank control group,the expression of phosphorylated NF-κB was increased in hanoxia-reoxygenation group,while decreased in adding Tongmai Xiaoban decoction group.Western Blot results showed that the expression of PPARa was decreased in hanoxia-reoxygenation group compare

关 键 词：通脉消斑汤 PPARΑ NF-κB 心肌细胞 实验研究 

分 类 号：R542.21[医药卫生—心血管疾病]