被动凝集法和化学发光法诊断儿童肺炎支原体感染的应用价值  

Application Value of Passive Agglutination Method and Chemiluminescence Method in Diagnosing Mycoplasma Pneumoniae Infection of Children

作　　者：容冰 苏春燕 RONG Bing;SU Chunyan(Laboratory Department,Gaozhou City Traditional Chinese Medicine Hospital,Gaozhou,Guangdong 525200)

机构地区：[1]高州市中医院检验科,广东高州525200

出　　处：《智慧健康》2024年第13期82-84,88,共4页Smart Healthcare

摘　　要：目的探讨被动凝集法和化学发光法诊断儿童肺炎支原体(MP)感染的价值。方法选取本院2021年4—12月收治的53呼吸道感染患儿作为研究对象,抽取患儿空腹静脉血3mL,采用被动凝集法、化学发光法进行检测,以免疫荧光法检测结果为金标准,分析动凝集法、化学发光法对儿童肺炎支原体感染的检出率以及对儿童肺炎支原体感染的诊断效能。结果以免疫荧光法为金标准,检测53例呼吸道感染患儿,其结果显示MP感染43例,检出率为43/53(81.13%),非MP感染为10例,检出率为10/53(18.87%);其中化学发光法与病原学判断标准一致性好(Kappa=0.870),被动凝集法与病原学判断标准一致性尚可(Kappa=-0.537);化学发光法检测肺炎MP感染的特异度(40.00%)、敏感度(69.77%)、阳性预测值(83.33%)、阴性预测值(23.53%)及诊断准确率(64.15%)均高于被动凝集法检测,两者之间差异有统计学意义(P<0.05)。结论被动凝集法、化学发光法均可对儿童肺炎支原体感染进行检查,化学发光法检测可提高对儿童MP感染早期的检测诊断率。Objective To explore value of passive agglutination and chemiluminescence methods in diagnose of mycoplasma pneumoniae(MP)infection of children.Methods The paper chose 53 children with respiratory tract infections in our hospital from April 2021 to December 2021 as research subjects,extracted 3ml of fasting venous blood,carried on detection with passive agglutination method and chemiluminescence method.Detection rate and diagnostic effect of dynamic agglutination method and chemiluminescence method for mycoplasma pneumoniae infection of children was analyzed with gold standard of immunofluorescence method.Results 43 cases of MP infection were detected among 53 children with respiratory tract infections,with detection rate of 43/53(81.13%),10 cases of non MP infection were detected,with detection rate of 10/53(18.87%).Chemiluminescence method showed good consistency with pathogen determination criteria(Kappa=0.870),while passive agglutination method showed acceptable consistency with pathogen determination criteria(Kappa=-0.537).Specificity(40.00%),sensitivity(69.77%),positive predictive value(83.33%),negative predictive value(23.53%)and diagnostic accuracy(64.15%)of chemiluminescence method for pneumonia MP infection was higher than passive agglutination method(P<0.05).Conclusion Both passive agglutination and chemiluminescence methods can detect mycoplasma pneumoniae infection of children,and chemiluminescence detection can improve early detection and diagnosis rate of children with MP infection.

关 键 词：被动凝集法 化学发光法 儿童肺炎 支原体感染 

分 类 号：R725.6[医药卫生—儿科]